CCR7 Receptor Expression in Mono-MAC-1 Cells: Modulation by Liver X Receptor α Activation and Prostaglandin E₂

Figure 5

Functional migration in response to PG and T0901317 is dependent on E and E receptors in MM-1 cells. (a) MM-1 cells were treated for 8 h (filled black) and 24 h (filled gray) with the indicated concentrations of 11-deoxy-PGE₁, 17-phenyl-trinor-PG, and butaprost. Total RNA was extracted and CCR7 transcripts were detected using real-time qPCR. Data represent mean ± SD of three independent experiments. Chemotaxis assays in response to 300 ng/mL CCL19 (b) or CCL21 (c) were performed using MM-1 cells stimulated for 48 h with 1 μM T0901317. The mean number of spontaneously migrating cells (that migrated to media alone) was subtracted from the number of cells that migrated in response to CCL19. Data represent mean ± SD of three independent experiments. , , , and .

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