EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells

Figure 2

LPS induces the production of EOLA1 and VCAM-1 in ECV304 cells. (a) ECV304 cells were treated with LPS (100 ng/mL) for various periods of time, 0, 3, 6, 12, and 24 h, and the total RNA was analyzed by RT-PCR to EOLA1. (b) The lysates of ECV304 cells were analyzed by Western blotting with antibodies to EOLA1. Data show one representative experiment out of three independent experiments. (c) ECV304 cells were stimulated with LPS (100 ng/mL) for the indicated periods of time and the supernatants were collected and assayed for VCAM-1 production by ELISA. Data represent the mean ± SD of three independent experiments.

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