Lipopolysaccharide from Rhodobacter sphaeroides Attenuates Microglia-Mediated Inflammation and Phagocytosis and Directs Regulatory T Cell Response

Figure 7

Treg cells confer tolerogenic potential to microglia. CD4⁺ T cells were treated with recombinant IL-2 in presence or absence of IL-10 for 72 hrs and the supernatants were used for pretreatment of microglia followed by stimulation with LPS; production of nitric oxide (a) and TNF-α (b) into the culture medium was analysed by Griess reagent and ELISA, respectively. The error bars represent the mean ± SEM from three independent experiments ( versus control and versus LPS). T cells sup.: supernatant from T cells; UT: untreated; rIL-2: recombinant IL-2; rIL-10: recombinant IL-10.

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