Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model

Figure 4

Flow cytometric analysis of lung and splenic T lymphocyte subsets in a murine model of ethanol exposition for 2 weeks, surgery, and pulmonary infection. (a) Flow cytometric analysis of CD3+ cells in the lung in ethanol-treated (ETOH) and saline-treated controls (NaCl) at 24 hours or 48 hours after pulmonary infection with Klebsiella pneumoniae. (b) Flow cytometric analysis of CD4+ cells in the lung in ethanol-treated (ETOH) and saline-treated controls (NaCl) at 24 hours or 48 hours after pulmonary infection with Klebsiella pneumoniae. (c) Flow cytometric analysis of CD8⁺ cells in the lung in ethanol-treated (ETOH) and saline-treated controls (NaCl) at 24 hours or 48 hours after pulmonary infection with Klebsiella pneumoniae. All cell counts are given as number of cells per 50.000 lymphocytes. when comparing ethanol treatment and saline-treated controls at the same time-point (ethanol-dependent effects); when compared to time- and treatment-matched control (infection-dependent effects); when comparing 24 hours to 48 hours (time-dependent effects); data are presented as mean ± SEM, Mann–Whitney U test ( each group). CD: cluster of differentiation; ETOH: ethanol treatment; K. pneumoniae: pulmonary infection with Klebsiella pneumoniae.

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