Research Article | Open Access
Felix Agyei Ampadu, Eric Boakye-Gyasi, Newman Osafo, Charles Kwaku Benneh, Edmund Ekuadzi, Eric Woode, "Antipleuritic and Vascular Permeability Inhibition of the Ethyl Acetate-Petroleum Ether Stem Bark Extract of Maerua angolensis DC (Capparaceae) in Murine", International Journal of Inflammation, vol. 2018, Article ID 6123094, 12 pages, 2018. https://doi.org/10.1155/2018/6123094
Antipleuritic and Vascular Permeability Inhibition of the Ethyl Acetate-Petroleum Ether Stem Bark Extract of Maerua angolensis DC (Capparaceae) in Murine
Maerua angolensis has been used traditionally in the management of pain, arthritis, and rheumatism in Ghana and Nigeria but no scientific evidence is currently available to give credence to its folkloric use. The aim of this study was therefore to evaluate the anti-inflammatory effects of a stem bark extract of Maerua angolensis DC (MAE) in acute inflammatory models. The effects of MAE (30-300 mg kg−1) on neutrophil infiltration, exudate volume, and endogenous antioxidant enzymes in lung tissues and lung morphology were evaluated with the carrageenan induced pleurisy model in Sprague Dawley rats. The effects of MAE (30-300 mg kg−1) on vascular permeability were also evaluated in the acetic acid induced vascular permeability in ICR mice. MAE significantly reduced neutrophil infiltration, exudate volume, and lung tissue damage in carrageenan induced pleurisy. MAE increased the activities of antioxidant enzymes glutathione, superoxide dismutase, and catalase in lung tissues. The extract was also able to reduce myeloperoxidase activity and lipid peroxidation in lung tissues in carrageenan induced rat pleurisy. Vascular permeability was also attenuated by the extract with marked reduction of Evans blue dye leakage in acetic acid induced permeability assay. The results indicated that Maerua angolensis is effective in ameliorating inflammation induced by carrageenan and acetic acid. It also has the potential of increasing the activity of endogenous antioxidant enzymes.
Inflammation is a defensive response to injury to curb further damage and to also initiate tissue repair . It is currently understood that inflammation is part of the nonspecific immune response that occurs in reaction to any type of cellular injury which may be mechanical (e.g., contusion or abrasion), chemical (e.g., toxins, acid, and alkaline), physical (e.g., extreme heat or cold), microbes (e.g., bacteria, virus, and parasites), necrotic tissue, oxidative stress, and/or immunological reactions. In the inflammatory response there are increased blood flow, cellular metabolism, vasodilatation, release of soluble mediators, fluid extravasation, and cellular influx. Under normal conditions, inflammation is self-limiting but in some disease states there is persistent injury and further cell damage which then leads to chronic inflammatory disorders [2, 3]
Maerua angolensis is a tropical plant that is widespread in the savannah area of tropical Africa to South Africa and Swaziland . Maerua angolensis has a long history of use in traditional medicine to manage various conditions such as psychosis, epilepsy, pain, gout, diabetes, peptic ulcer, diarrhea, and arthritis in Nigeria and other West African countries [5, 6]. Most of the medicinal plants from folklore have not been assessed for their toxicity, mechanisms of action, and interactions with food and other medicines . Hence, it is prudent to conduct further studies into medicinal plants to obtain new data on indications and safety profile.
The cellular and molecular mechanism of the carrageenan induced pleurisy is well characterized . The early phase of the carrageenan induced inflammation is related to the production of histamine, leukotrienes, platelet-activating factor, and possibly cyclooxygenase products, while the delayed phase of the carrageenan-induced inflammatory response has been linked to neutrophil infiltration and the production of neutrophil-derived free radicals and oxidants, such as hydrogen peroxide, superoxide, and hydroxyl radical, as well as to the release of other neutrophil-derived mediators [9, 10] that causes tissue damage. As neutrophils play major roles in acute and chronic inflammation, identification of compounds that inhibit its infiltration to the site of inflammation may result in new paradigms in the management of inflammatory diseases [11, 12]. The carrageenan pleurisy model allows the assessment and quantification of multiple inflammatory parameters. After vasodilation in the initial phase inflammation endothelial cells in local capillary beds contract, generating spaces between the cells which substantially increase vascular permeability. Vascular permeability enhances fluid and cellular extravasation which results in localized edema observed in the inflammatory response. It has been well established that the intraperitoneal injection of acetic acid greatly enhances vascular permeability and facilitates the vascular leakage of Evans blue dye . The use of Evans blue dye as an in vivo marker through vascular permeability facilitates the investigation of the effect of pathological changes in various disorders mainly, immunological disorders, inflammatory disorders, and cardiovascular diseases like atherosclerosis myocardial infarction, cancers, and others.
2.1. Plant Collection and FT-IR Analysis of Extract
The stem bark of Maerua angolensis was collected from the rocky areas of Kwahu Tafo to Nkyenenkyene road, Eastern Region, Ghana (6.415360 N 0.363160 W), and was authenticated by Professor Kofi Annan, Department of Herbal Medicine, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, Kumasi. A voucher specimen (KNUST/FP/12/051) was deposited at the herbarium at the faculty.
The stem bark was room-dried for seven days and pulverized into fine powder. The powder was extracted with a mixture of petroleum ether-ethyl acetate (1:1) at 25°C to obtain an extract labelled as MAE throughout this study. The extract was then concentrated under reduced pressure at 60°C using a rotary evaporator (Rotavapor R-215, BÜCHI Labortechnik AG, Flawil, Switzerland) into green viscous mass. It was further dried in a hot air oven at 50°C for one week and then kept in a freezer (-20°C) until use (yield = 0.47 % w/w).
In order to identify possible functional groups that may be present in the extract, a triplicate FT-IR (PerkinElmer UATR Two) spectum was generated and baseline corrected. The spectra between 400–1400 cm−1 are usually considered as the unique region for every compound/compound mixtures and hence can be used for identification and quality control. The characteristic spectra (Figure 1) in the region from 400-1400 cm−1 were as a fingerprint region for subsequent comparison of future extracts.
Sprague Dawley rats (200–220 g) and Imprint Control Region (ICR) mice (20-30 g) were procured from Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, and housed in the animal facility of the Department of Pharmacology, KNUST, Kumasi, Ghana. The animals were kept in stainless steel cages, with wood shavings as bedding, fed with normal pellet diet and water available ad libitum. Sample size of 5-6 animals per group was utilized throughout the study, following guidelines according to National Institute of Health Guidelines for the Care and Use of Laboratory Animals (NIH, Department of Health and Human Services publication no. 85-23, revised 1985). All procedures used in this study were approved by the Department of Pharmacology Ethics Committee, College of Health Sciences (CoHS), KNUST, Kumasi, Ghana.
2.3. Chemicals and Reagents
Diclofenac sodium was purchased from Troge Medical GmbH, Hamburg, Germany. Phosphate buffered saline (PBS) was obtained from Gibco, Karlsruhe, Germany; methanol, ethanol, and sodium bicarbonate were obtained from Fisher Scientific, UK; acetic acid was obtained from BDH, Poole, England; trichloroacetic acid and haematoxylin and eosin Y were obtained from Abbey Color, Philadelphia, USA; xylene, formaldehyde, thiobarbituric acid, EDTA, monopotassium dihydrogen phosphate, dipotassium monohydrogen phosphate, monosodium phosphate, disodium phosphate, λ-carrageenan, o-dianisidine dihydrochloride, and Evans blue dye were obtained from Sigma-Aldrich, St. Louis, MO, USA.
2.4. Carrageenan Induced Rat Pleurisy
Rats (200-220 g) (n=6) were anesthetized with ether (0.25 mL in a 3 L container) open-drop method and subjected to a skin incision at the left sixth intercostal space. The underlying muscle was cut open, and 1% w/v λ-carrageenan suspension (0.1 mL) in normal saline was injected into the pleural space [14, 15]. The skin opening was closed with a stitch, and the rats were allowed to recover. The extract MAE (30, 100, and 300 mg kg−1, p.o.) or vehicle (10 mL kg−1, p.o.) and diclofenac (10, 30, and 100 mg kg−1, i.p.) were given 1 h and 30 min, respectively, before the injection of carrageenan. The rats were then sacrificed with excess of ether 6 h after carrageenan injection. The pleural cavity was opened and washed with 2 mL of saline solution containing 0.1% of EDTA. The pleural fluids were then aspirated and the volumes quantified (mL). The actual exudate volume was determined by subtracting the volume of solution injected (2 mL) from the total volume of fluid aspirated. Pleural fluids tainted with blood were excluded. The mobilized neutrophils in the exudate were quantified using an automated analyzer (ABX micros 60-Horiba, Irvine (CA), USA).
2.5. Histological Examination
Lung biopsies were taken 6 h after injection of carrageenan. Lung sections were collected and stored in 10% buffered formalin. The lung tissues were dehydrated with graded ethanol, embedded in paraffin, blocked, and sectioned. The sections were stained with hematoxylin and eosin and examined under light microscopy  (Axioscope Zeiss Microscope, Carl Zeiss Microimaging, Heidelberg, Germany). Damage to lung tissues was assessed by light microscopy . In each treatment group, six random fields of view were analyzed by observers unaware of the treatment protocols. The degree of microscopic lung damage induced by carrageenan was assessed. Histological slides were scored according to the following parameters: hyperaemia, oedema, alveolar septal thickening, and neutrophil infiltration . The degree of the disorganization was quantified on a scale of 0–4 (i.e., 0: not present, 1: very mild, 2: mild, 3: moderate, and 4: extensive).
2.6. Myeloperoxidase (MPO) Activity
Myeloperoxidase activity is an index of neutrophil accumulation. The influence of MAE on MPO activity in lung tissues was measured according to the method described by Bradley et al. [19, 20]. The assay mixture consisted of 0.3 mL 0.1 M phosphate buffer (pH 6.0), 0.3 mL 0.01 M H2O2, 0.5 mL 0.02 M o-dianisidine (freshly prepared) in deionized water, and 10 μL lung homogenate supernatant in a final volume of 3.0 mL. The supernatant was added last and the change in absorbance at 460 nm was monitored every 1 min for 10 min with a microplate reader (Synergy H1 Multi-Mode plate reader, Winooski, VT, USA). All measurements were carried out in triplicate. MPO activity was explained as one unit that increases absorbance at a rate of 0.001 min−1 and specific activity was expressed as units/mg protein.
2.7. Glutathione Assay
Aliquots 0.1 mL of 10 % tissue homogenate were mixed with 2.4 mL of 0.02 M EDTA solution and kept on ice bath for 10 min. Then 2 mL of distilled water and 0.5 mL of trichloroacetic acid (TCA) 50 % (w/v) were centrifuged at 3000 × g for 20 min at 4°C to remove precipitate. The supernatants, 1 mL, were then mixed with 2.0 mL of Tris buffer (0.4 M, pH 8.9) and 0.05 mL of 5’-dithiobisnitrobenzoic acid (DTNB) solution; Ellman’s reagent (10 mM) was added and swirled carefully. The absorbance was measured at 412 nm against a reagent blank with no homogenate after addition of DTNB and incubation at room temperature for 5 minutes [21, 22]. Glutathione was then quantified from a glutathione standard curve and expressed as μM mg−1 of protein.
2.8. Catalase Activity (CAT)
Medium consists of 130 L 50 mM potassium buffer (pH 7.0) and enzyme extract, 65 L of 10 mM H2O2. The blank had 65 L of the potassium phosphate and 130 L of sample. The concentration of H2O2 was estimated from the absorbance using the following equation: where 39.4 mol−1cm−1 is the molar extinction coefficient for H2O2. CAT activity was expressed as U mg−1 protein.
2.9. Superoxide Dismutase Activity (SOD)
SOD activity was determined as described by Misra and Fridovich [25, 26]. It is based on the ability of SOD to inhibit autoxidation of adrenaline to adrenochrome. 0.5 mL of tissue homogenate, 0.75 mL of ethanol, and 0.15 mL of chloroform (4°C) were combined in mixture. The mixture was then centrifuged at 2000 rpm for 20 min. Subsequently, 0.5 mL of supernatant, 0.5 mL of 0.6 mM EDTA solution, and 1 mL of carbonate bicarbonate buffer (0.1 M, pH 10.2) were added. The reaction was commenced by adding 0.05 mL of 1.3 mM adrenaline and the increase in absorbance at 480 nm due to the adrenochrome formation was measured with a microplate reader (Synergy H1 Multi-Mode plate reader, Winooski, VT, USA). One unit of SOD activity was defined as the amount of protein causing 50% inhibition of the autoxidation of adrenaline at 25°C.
2.10. Malondialdehyde Measurement (MDA)
Lipid peroxidation is an important marker of oxidative stress in pleuritis. Carrageenan induced pleurisy and lipid peroxidation (as analyzed by MDA) have been previously associated . The extent of lipid peroxidation in lung tissues was assessed by measuring MDA as described by Heath and Packer [28, 29]. 3 mL of 20% trichloroacetic acid containing 0.5% thiobarbituric acid was added to a 1 mL aliquot of lung homogenate supernatant in a test tube.
The mixture obtained was heated in a water bath (95°C) for 30 min and then allowed to cool. The test tube was then centrifuged at 10,000×g for 10 min, and the absorbance of the supernatant at 532 nm was measured. The value for the nonspecific absorption at 600 nm was subtracted from the 532 nm reading. The concentration of MDA was calculated using MDA's extinction coefficient of 155 mM−1cm−1.
2.11. Acetic Acid Induced Vascular Permeability
The acetic acid-induced vascular permeability test with slight modifications was conducted as previously described by Whittle [30, 31]. Groups of mice (25-30 g) (n=6) were treated with either MAE (30, 100, and 300 mg kg−1, p.o.), diclofenac (30 mg kg−1, i.p.), or vehicle. 1 h after treatments, each mouse was injected intravenously with 2 % Evan’s blue solution at 0.1 mL 10 g−1 body weight through the tail vein. 10 min afterwards, each mouse received 0.6 % acetic acid solution intraperitoneally at 0.1 mL 10 g−1 body weight. 30 min after acetic acid injection, the mice were sacrificed and peritoneal cavity washed three times with saline (10 mL). Saline washes were centrifuged for 5 min at 3500 rpm. The supernatants were collected and their absorbance was measured at 590 nm with a plate reader (Synergy H1 Multi-Mode plate reader, Winooski, VT, USA). Evans blue extravasation was enumerated from a standard curve and expressed in μg.
2.12. Statistical Analysis
The experimental data was expressed as mean ± SEM. Significance of difference among various treated groups and control group was analyzed by means of one-way analysis of variance followed by Dunnett’s multiple comparison test with GraphPad Prism for Windows version 5.01 (GraphPad Software, San Diego, CA, USA). P value less than 0.05 was considered significant.
3.1. Effects of Extract on Carrageenan Induced Pleurisy
Injection of carrageenan into the pleural cavity of rats elicited an inflammatory response within 6 h, characterized by the accumulation of fluid that contained large numbers of inflammatory cells as shown in Figure 2. MAE (300 mg kg−1) significantly inhibited the inflammatory response decreasing of exudate formation (F7,20=10.84, P<0.0001) and neutrophil infiltration (F7,20=8.86, P<0.0001) with a maximal effect of 64.2% and 90.9%, respectively (Figure 2).
MAE (100 mg kg−1) also significantly reduced exudate formation (F7,20=9.58, P<0.0001) and neutrophil infiltration (F7,20=8.41, P<0.0001) with a maximal effect at 57.1% and 86.5%, respectively. Diclofenac (100 mg kg−1) used as a standard anti-inflammatory agent also significantly reduced exudate formation (F7,20=14.96, P<0.0001) and neutrophil infiltration (F7,20=9.18, P<0.0001) almost completely with a maximal effect of 75% and 96.6%, respectively (Figure 2).
From the ED50 calculated from the dose response curves (Figures 3(a) and 3(b)), MAE was found to be approximately 3.8× less potent (ED50=12.26±3.90) than diclofenac (ED50=3.22±4.45) in reducing neutrophil infiltration and 3.5x less potent (ED50=60.57±15.82) than diclofenac (ED50=17.13±17.11) in reducing exudate formation.
Histological examinations aid in the identification and assessment of microscopic morphological changes in cells and tissues. Vehicle only treated rats showed normal lung architecture (Figure 4(a)) with little or no signs of neutrophil infiltration, oedema, hyperaemia, and alveolar septal thickening. Carrageenan treated rats that received no drug treatment showed extensive disorganization of alveolar structures (Figure 4(b)) with significant presence of neutrophils, oedema, hyperaemia, and alveolar septal thickening (Figure 5). When rats were treated with MAE (300 mg kg−1), there was reduced alveolar structural disorganization (Figure 4(d)). This presented with a significantly (P<0.0001) suppressed neutrophil infiltration, oedema, hyperaemia, and alveolar septal thickening (Figure 5).
Rats treated with a diclofenac and carrageenan (Figure 4(c)) showed significant (P<0.0001) suppression of neutrophil infiltration with minor or no oedema, hyperaemia, and alveolar septal thickening when compared with carrageenan only treated rats (Figure 5).
3.3. Enzyme Assays
MPO and CAT activity and MDA level in the lung tissue were increased after intrapleural injection of carrageenan. Compared to carrageenan only treated tissues, there was a significant (P<0.05) reduction in MPO activity in MAE (30, 100, and 300 mg kg−1) treated rats. Levels of MDA also reduced significantly in MAE (100 and 300 mg kg−1) treated rats. MAE also increased CAT activity significantly at all doses (Figures 6(a), 9(a), and 10(a)). Similar reductions in MPO activity and MDA levels were observed at 30 and 100 mg kg−1 of diclofenac.
Additionally, catalase activity was significantly increased at all tested doses ((10-100 mg kg−1) of diclofenac. From the dose response analysis, the potency ratios of diclofenac and MAE with respect to MPO activity, CAT activity, and MDA expression are 2.1, 12.8, and 1.2, respectively.
GSH and SOD present in vehicle only treated tissues significantly decreased after carrageenan injection. Compared to carrageenan only treated lung tissues, there was a significant increase in GSH levels and SOD activity in 30, 100, and 300 mg kg−1 MAE (P<0.0001) treated lung tissues (Figures 7(a) and 8(a)). Similarly, the positive control diclofenac also showed significant increase in GSH levels and SOD activity at all doses (10-100 mg kg−1) compared to carrageenan only treated tissues (P<0.0001) (Figures 7(a) and 8(a)). From the ED50 calculated from the dose response curves (Figures 7(b) and 8(b)), MAE is approximately 2.4x more potent than diclofenac in increasing both GSH expression and SOD activity.
3.4. Vascular Permeability
Injection of acetic acid into the peritoneum of mice previously injected intravenously with 2% Evans blue dye exhibited an inflammatory response after 30 min, characterized by the extravasation of Evans blue dye into the peritoneal cavity of the mice. The effect of MAE on dye extravasation in peritoneal fluid after acetic acid challenge was determined with an Evans blue standard curve as shown in Figure 11(a). There was a significant decrease in dye leakage in mice treated with MAE at 100 mg kg−1 (73% inhibition, P<0.005) and 300 mg kg−1 (83% inhibition, P<0.0025) when compared to controls. Diclofenac at a dose of 30 mg kg−1 also caused a significant decrease in dye leakage (78% inhibition, P<0.003) when compared to controls (Figure 11(b)).
In this study, the anti-inflammatory properties of an ethyl acetate-petroleum ether (1:1) stem bark extract of M. angolensis (MAE) were evaluated. The carrageenan induced rat pleurisy model, which is useful for evaluating anti-inflammatory drug candidates, especially those derived from natural products and the acetic acid induced vascular permeability models were employed. This model is characterized by an early phase (4 h after carrageenan administration), in which leukocyte migration to the pleural cavity and the lungs is significantly enhanced and histological changes occurs [14, 30]. This early response is associated with a marked activation of the NF-κB and p38 MAPK pathways, which leads to a massive generation of proinflammatory mediators such as TNF-α, IL-1β, and NO, in addition to the increased activity of important proinflammatory enzymes such as adenosine deaminase (ADA) and MPO [8, 32]
Cellular infiltration was observed when carrageenan was injected into the pleural cavity of rats. There was an inflammatory reaction associated with exudation of fluids into the pleural space accompanied by a high influx of neutrophils. This ultimately leads to the increased levels of prostaglandin E2, TNF-α and IL-1β, ROS, and lipid peroxidation . Cell migration occurs as a consequence of several processes including adhesion and cell mobility .
MAE-treated groups showed significantly fewer neutrophils in the pleural exudates than the controls, suggesting the inhibition of neutrophil infiltration which may be due to inhibition of rolling and adhesion of neutrophils, which impaired neutrophil migration from blood vessels. MAE produced a dose dependent inhibition in the influx of neutrophils and exudate volume into the pleural cavity similar to diclofenac. PGE2 is the mediator primarily responsible for the exudation that occurs in carrageenan induced pleurisy via EP2 and EP3 receptors [35, 36].
Hence, inhibition of EP2 and EP3 receptors might contribute to the anti-inflammatory activity of MAE. Exudate formation also results from elevated NO levels produced by activation of inducible nitric oxide synthase (iNOS) in endothelial and inflammatory cells [37, 38]. Since MAE was able to reduce exudate levels, it may be involved in the blockade of iNOS activation. Additionally, the neutrophil decrease that was observed was followed by a reduction in MPO activity . MPO is a proinflammatory enzyme found in cytoplasmic granules of neutrophils which leads to the generation of cytotoxic compounds such as hypochlorous acid and tyrosyl radicals from hydrogen peroxide and tyrosine, respectively [40, 41].
Oedema is just one component of the inflammatory response; increased vascular permeability also plays a significant role. Hence, to further investigate the inhibitory effect of the extract on the prostaglandin, serotonin, and histamine pathways of inflammation, the role of the extract in acetic acid induced vascular permeability was investigated. In acetic acid-induced vascular permeability test, acetic acid challenge brings about increases in the level of mediators such as prostaglandins, serotonin, and histamine in peritoneal fluids, which in turn lead to vasodilation and an increase in vascular permeability [13, 42]. MAE dose-dependently attenuated the capillary permeability induced by acetic acid in mice. These findings suggest that the anti-inflammatory effect of MAE on the acute phase of inflammation might be associated with prevention of vasodilation and inhibition of the release of inflammatory mediators such as histamine and serotonin.
In acute inflammation, there is decreased activity of endogenous antioxidant enzymes (CAT, SOD, and GSH) and increased lipid peroxidation in tissues as a result of oxidative damage . CAT, SOD, and GSH also play a crucial role as protective enzymes. Schreck et al.  demonstrated that antioxidants inhibit nuclear factor kappa light-chain-enhancer of activated B cells (NF-kB), whereas ROS activate NF-κB, a transcription factor which activates the transcription of several genes involved in inflammation [45, 46]. The protective role of GSH against inflammatory diseases has been proven by depleting endogenous GSH with Buthionine sulfoximine (BSO) [47, 48] which resulted in aggravating effect on various models of inflammation including carrageenan induced pleurisy. CAT which is localized in subcellular organelles of peroxisomes catalyses the conversion of hydrogen peroxide to water and oxygen ; SOD in cells work in conjunction with H2O2-removing enzymes such as glutathione peroxidase (GPx) or CAT to prevent action of H2O2, which in turn inhibits the formation of hydroxyl radicals [50, 51]. Treatment with MAE increased the activities of CAT, SOD, and GSH. The above effects resulted in decreased lipid peroxidation from the low levels of MDA in MAE-treated rats thereby significantly reducing the severity of inflammation.
Histopathology can offer a pronounced structural peculiarity as a pragmatic, univocal, and decisively characteristic sign of an inflammatory process . Henceforth, histopathological studies on lung sections after carrageenan challenge were carried out which showed the extract was able to preserve normal alveolar architecture with reduced influx of neutrophils and oedema formation. The alveolar walls were markedly less thickened with less hyperaemia. MAE showed ability to attenuate lung injury induced by carrageenan and this is in agreement with Wilson et al. [53, 54] finding which established that materials that act on multiple proinflammatory mediators improve lung function in inflammation.
The ethyl acetate-petroleum ether stem bark extract of Maerua angolensis has anti-inflammatory activity in acute inflammation by attenuating carrageenan induced pleurisy and decreasing acetic acid-induced vascular permeability. The extract has also exhibited significant in vivo antioxidant activity, which may contribute to its anti-inflammatory activity.
The data used to support the findings of this study are available from the corresponding author upon request.
Conflicts of Interest
Authors declare no conflicts of interest.
All authors participated in the conception and design of the study. Eric Boakye-Gyasi and Felix Agyei Ampadu participated in data collection. Eric Boakye-Gyasi, Felix Agyei Ampadu, Newman Osafo, Charles Kwaku Benneh, and Eric Woode performed data management and analysis. All authors collaborated on the initial drafts of the manuscript. All authors contributed further to interpretation of the results and manuscript edits. All authors read and approved the final manuscript.
The authors appreciate the technical assistance of Mr. Thomas Ansah, Mr. Edmond Dery, and technical staff of the Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
- L. Ferrero-Miliani, O. H. Nielsen, P. S. Andersen, and S. E. Girardin, “Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation,” Clinical and Experimental Immunology, vol. 147, no. 2, pp. 227–235, 2007.
- D. W. Gilroy, T. Lawrence, M. Perretti, and A. G. Rossi, “Inflammatory resolution: new opportunities for drug discovery,” Nature Reviews Drug Discovery, vol. 3, no. 5, pp. 401–416, 2004.
- C. N. Serhan and J. Savill, “Resolution of inflammation: the beginning programs the end,” Nature Immunology, vol. 6, no. 12, pp. 1191–1197, 2005.
- R. A. Mothana, U. Lindequist, R. Gruenert, and P. J. Bednarski, “Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra,” BMC Complementary and Alternative Medicine, vol. 9, article 7, 2009.
- G. E. Wickens and H. M. Burkill, “The useful plants of west tropical Africa,” Royal Botanic Gardens, Kew, vol. 1–3, no. 2, pp. 471-472, 1995.
- R. Ayo, O. Audu, J. Amupitan, and E. Uwaiya, “Phytochemical screening and antimicrobial activity of three plants used in traditional medicine in Northern Nigeria,” Journal of Medicinal Plants Research, pp. p191–197, 2013.
- M. M. Iwu, Handbook of African Medicinal Plants, CRC Press, Boca Raton, Fla, USA, 2nd edition, 2014.
- T. S. Frode and Y. S. Medeiros, “Myeloperoxidase and adenosine-deaminase levels in the pleural fluid leakage induced by carrageenan in the mouse model of pleurisy,” Mediators of Inflammation, vol. 10, pp. 1–5, 2001.
- V. R. Sunil, J. Shen, K. Patel-Vayas, A. J. Gow, J. D. Laskin, and D. L. Laskin, “Role of reactive nitrogen species generated via inducible nitric oxide synthase in vesicant-induced lung injury, inflammation and altered lung functioning,” Toxicology and Applied Pharmacology, vol. 261, no. 1, pp. 22–30, 2012.
- R. G. Ferreira, T. C. Matsui, L. F. Gomides et al., “Niacin inhibits carrageenan-induced neutrophil migration in mice,” Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 386, no. 6, pp. 533–540, 2013.
- T. C. Matsui, G. M. E. Coura, I. S. F. Melo et al., “Nicorandil inhibits neutrophil recruitment in carrageenan-induced experimental pleurisy in mice,” European Journal of Pharmacology, vol. 769, pp. 306–312, 2015.
- H. R. Jones, C. T. Robb, M. Perretti, and A. G. Rossi, “The role of neutrophils in inflammation resolution,” Seminars in Immunology, vol. 28, no. 2, pp. 137–145, 2016.
- R. B. Nidavani, A. M. Mahalakshmi, and M. Shalawadi, “Vascular permeability and evans blue dye: A physiological and pharmacological approach,” Journal of Applied Pharmaceutical Science, vol. 4, no. 11, pp. 106–113, 2014.
- J. S. Bus, A. Vinegar, and S. M. Brooks, “Biochemical and physiologic changes in lungs of rats exposed to a cadmium chloride aerosol,” The American Review of Respiratory Disease, vol. 118, no. 3, pp. 573–580, 1978.
- R. Vinegar, J. F. Truax, J. L. Selph, and F. A. Voelker, “Pathway of onset, development, and decay of carrageenan pleurisy in the rat,” Federation Proceedings, vol. 41, no. 9, pp. 2588–2595, 1982.
- S. F. Ahmad, S. M. Attia, S. A. Bakheet et al., “Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines,” Inflammation, vol. 38, no. 2, pp. 846–857, 2015.
- B. V. Patel, M. R. Wilson, and M. Takata, “Resolution of acute lung injury and inflammation: a translational mouse model,” European Respiratory Journal, vol. 39, no. 5, pp. 1162–1170, 2012.
- Y.-H. Hsieh, J.-S. Deng, H.-P. Pan, J.-C. Liao, S.-S. Huang, and G.-J. Huang, “Sclareol ameliorate lipopolysaccharide-induced acute lung injury through inhibition of MAPK and induction of HO-1 signaling,” International Immunopharmacology, vol. 44, pp. 16–25, 2017.
- P. P. Bradley, D. A. Priebat, R. D. Christensen, and G. Rothstein, “Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker,” Journal of Investigative Dermatology, vol. 78, no. 3, pp. 206–209, 1982.
- G. Otulakowski, D. Engelberts, H. Arima et al., “α-Tocopherol transfer protein mediates protective hypercapnia in murine ventilator-induced lung injury,” Thorax, vol. 72, no. 6, pp. 538–549, 2017.
- M. Ellman, “A spectrophotometric method for determination of reduced glutathione in tissues,” AnalytBiochem, vol. 74, pp. 214–226, 1959.
- F. Petronilho, D. Florentino, F. Silvestre et al., “Ebselen Attenuates Lung Injury in Experimental Model of Carrageenan-Induced Pleurisy in Rats,” Inflammation, vol. 38, no. 4, pp. 1394–1400, 2015.
- H. Aebi, “ Catalase in vitro,” Methods in Enzymology, vol. 105, pp. 121–126, 1984.
- E. Ekuadzi, R. P. Biney, C. K. Benneh, B. Osei Amankwaa, and J. Jato, “Antiinflammatory properties of betulinic acid and xylopic acid in the carrageenan-induced pleurisy model of lung inflammation in mice,” Phytotherapy Research, vol. 32, no. 3, pp. 480–487, 2018.
- H. P. Misra and I. Fridovich, “The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.,” The Journal of Biological Chemistry, vol. 247, no. 10, pp. 3170–3175, 1972.
- d. A. Fabiola, d. O. Emiliano, R. S. Mairim et al., “Anti-inflammatory property and redox profile of the leaves extract from Morinda citrifolia L.,” Journal of Medicinal Plants Research, vol. 9, no. 24, pp. 693–701, 2015.
- H. H. Draper, E. J. Squires, H. Mahmoodi, J. Wu, S. Agarwal, and M. Hadley, “A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials,” Free Radical Biology & Medicine, vol. 15, no. 4, pp. 353–363, 1993.
- R. L. Heath and L. Packer, “Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation,” Archives of Biochemistry and Biophysics, vol. 125, no. 1, pp. 189–198, 1968.
- S. D. Müller, D. Florentino, C. F. Ortmann et al., “Anti-inflammatory and antioxidant activities of aqueous extract of Cecropia glaziovii leaves,” Journal of Ethnopharmacology, vol. 185, pp. 255–262, 2016.
- B. A. Whittle, “The use of changes in capillary permeability in mice to distinguish between narcotic and non-narcotic analgesic,” British Journal of Pharmacology and Chemotherapy, vol. 22, pp. 246–253, 1964.
- Z. Zhao, J. Xiao, J. Wang, W. Dong, Z. Peng, and D. An, “Anti-inflammatory effects of novel sinomenine derivatives,” International Immunopharmacology, vol. 29, no. 2, pp. 354–360, 2015.
- S. Marzocco, R. Di Paola, I. Serraino et al., “Effect of methylguanidine in carrageenan-induced acute inflammation in the rats,” European Journal of Pharmacology, vol. 484, no. 2-3, pp. 341–350, 2004.
- J. R. Vane and R. M. Botting, “Anti-inflammatory drugs and their mechanism of action,” Inflammation Research, vol. 47, supplement 2, pp. S78–S87, 1998.
- J. Akaogi, T. Nozaki, M. Satoh, and H. Yamada, “Role of PGE2 and EP receptors in the pathogenesis of rheumatoid arthritis and as a novel therapeutic strategy,” Endocrine, Metabolic & Immune Disorders—Drug Targets, vol. 6, no. 4, pp. 383–394, 2006.
- V. C. Jones, M. A. Birrell, S. A. Maher et al., “Role of EP2 and EP4 receptors in airway microvascular leak induced by prostaglandin E2,” British Journal of Pharmacology, vol. 173, no. 6, pp. 992–1004, 2016.
- S. Ghosh and S. C. Erzurum, “Nitric oxide metabolism in asthma pathophysiology,” Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1810, no. 11, pp. 1008–1016, 2011.
- S. K. Biswas, “Does the Interdependence between Oxidative Stress and Inflammation Explain the Antioxidant Paradox?” Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 5698931, pp. 1–9, 2016.
- F. Agyei Ampadu, “Anti-inflammatory and antioxidant effects of stem bark extracts of maerua angolensis dc (capparaceae),” 2016.
- B. Pulli, M. Ali, R. Forghani et al., “Measuring myeloperoxidase activity in biological samples,” PLoS ONE, vol. 8, no. 7, Article ID e67976, 2013.
- J. Huang, A. Milton, R. D. Arnold et al., “Methods for measuring myeloperoxidase activity toward assessing inhibitor efficacy in living systems,” Journal of Leukocyte Biology, vol. 99, no. 4, pp. 541–548, 2016.
- G. M. Nardi, J. M. Siqueira Junior, F. Delle Monache, M. G. Pizzolatti, K. Ckless, and R. M. Ribeiro-do-Valle, “Antioxidant and anti-inflammatory effects of products from Croton celtidifolius Bailon on carrageenan-induced pleurisy in rats,” Phytomedicine, vol. 14, no. 2-3, pp. 115–122, 2007.
- I. Posadas, M. Bucci, F. Roviezzo et al., “Carrageenan-induced mouse paw oedema is biphasic, age-weight dependent and displays differential nitric oxide cyclooxygenase-2 expression,” British Journal of Pharmacology, vol. 142, no. 2, pp. 331–338, 2004.
- R. Schreck, P. Rieber, and P. A. Baeuerle, “Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-κB transcription factor and HIV-1,” EMBO Journal, vol. 10, no. 8, pp. 2247–2258, 1991.
- P. A. Baeuerle and T. Henkel, “Function and activation of NF-κB in the immune system,” Annual Review of Immunology, vol. 12, pp. 141–179, 1994.
- J. Watson, “Oxidants, antioxidants and the current incurability of metastatic cancers,” Open Biology, vol. 3, no. 1, Article ID 120144, 2013.
- S. Cuzzocrea, G. Costantino, B. Zingarelli, E. Mazzon, A. Micali, and A. P. Caputi, “The protective role of endogenous glutathione in carrageenan-induced pleurisy in the rat,” European Journal of Pharmacology, vol. 372, no. 2, pp. 187–197, 1999.
- H. P. Bordini, J. L. Kremer, T. R. Fagundes et al., “Protective effect of metformin in an aberrant crypt foci model induced by 1,2-dimethylhydrazine: Modulation of oxidative stress and inflammatory process,” Molecular Carcinogenesis, vol. 56, no. 3, pp. 913–922, 2017.
- A. Agarwal and S. A. Prabakaran, “Mechanism, measurement and prevention of oxidative stress in male reproductive physiology,” Indian Journal of Experimental Biology, vol. 43, no. 11, p. 963, 2005.
- J. Limón-Pacheco and M. E. Gonsebatt, “The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress,” Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 674, no. 1-2, pp. 137–147, 2009.
- P. R. Kvietys and D. N. Granger, “Role of reactive oxygen and nitrogen species in the vascular responses to inflammation,” Free Radical Biology & Medicine, vol. 52, no. 3, pp. 556–592, 2012.
- A. Soren, N. S. Cooper, and T. R. Waugh, “The nature and designation of osteoarthritis determined by its histopathology,” Clinical and Experimental Rheumatology, vol. 6, no. 1, pp. 41–46, 1988.
- S. Gopi, A. Amalraj, S. Jude et al., “Preparation, characterization and anti-colitis activity of curcumin-asafoetida complex encapsulated in turmeric nanofiber,” Materials Science and Engineering C: Materials for Biological Applications, vol. 81, pp. 20–31, 2017.
- M. R. Wilson, K. P. O'Dea, D. Zhang, A. D. Shearman, N. Van Rooijen, and M. Takata, “Role of lung-marginated monocytes in an in vivo mouse model of ventilator-induced lung injury,” American Journal of Respiratory and Critical Care Medicine, vol. 179, no. 10, pp. 914–922, 2009.
- M. R. Wilson, J. E. Petrie, M. W. Shaw et al., “High-fat feeding protects mice from ventilator-induced lung injury, via neutrophil-independent mechanisms,” Critical Care Medicine, vol. 45, no. 8, pp. e831–e839, 2017.
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