-Equol, a Major Isoflavone from Soybean, Inhibits Nitric Oxide Production in Lipopolysaccharide-Stimulated Rat Astrocytes Partially via the GPR30-Mediated Pathway

Figure 6

Effect of ER antagonist/agonist on LPS-induced NO production. Cultured astrocytes were treated with LPS (1 μg/ml) and S-equol (50 μM) in the presence of ICI 182.780 (an intracellular ER antagonist; 1 μM) or G-15 (a GPR30 antagonist; 1 μM) for 24 h, and NO production was evaluated as described in Figure 3. Some cultures were treated with LPS (1 μg/ml) and G-1 (a GPR30 agonist; 100 nM) for 24 h, and NO production was determined. Data are the mean ± SEM of 5 samples. ; significantly different from LPS. statistical difference between LPS + S-equol and LPS + S-equol + G-15. statistical difference between LPS + S-equol + ICI and LPS + S-equol + G-15.