IL-6/Illumina HumanMethylation27K DNA Analysis BeadChip
Among patients with lifetime depression, there was a significant inverse correlation between methylation of IL-6 and serum levels of IL-6 and CRP (Pearson r=-0.54, p=0.001 and Pearson r=-0.48, p=0.006, respectively.
A positive trend between the levels of SOCS-1 methylation and CRP levels was observed (R2=0.1127, p=0.0278). Patients with serum IL-6 above median showed a significantly higher SOCS-1 methylation than the patients with serum IL-6 below median (p<0.001). Similar results were observed for TNF-α (p<0.001).
SOCS-1: Cancer, hepatic system diseases, ophthalmological diseases.
LY86 gene, 6 CpG sites / HumanMethylation 27 BeadChip and ThumanMethylation 450 BeadChip from Illumina
Age, sex, race, BMI and batch
They performed a principal component analysis to combine the six CpG sites into one score. The score of these CpG sites was significantly associated with fibrinogen (partial r=0.145, p<0.001) and CRP (partial r=0.114, p=0.005).
Plasma CRP levels were significantly associated with IL-6 promoter methylation (p=0.025). One interquartile range increase in plasma CRP was associated with a decrease in IL-6 methylation by 0.78% (95% CI: -1.47% to -0.1%).
Results showed correlations between LEPR methylation and concentration of LEP (r=-0.24, p=0.047). Also, between EEF2 methylation and concentration of TNF-α (r=0.24, p= 0.0408) and CRP (r=0.24, p=0.0457).
IPA: Inflammatory response, cardiovascular disease, reproductive system diseases.