The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages

Figure 10

The effects of lithium on expression of NF-κB signalling-related genes on Raw 264.7 cells activated with LPS. Cells were cultured in 60 mm cell culture dishes overnight at a density of 6 × 10⁶ cell/ml. Thereafter, cells were treated with 10 mM lithium, 10 μm/ml LPS, and a combination of lithium and LPS for 24 hrs. Total RNA isolation was accomplished with Qiagen Total RNA isolation kit; cDNA was synthesised with RT² first-strand kit. RT² profiler PCR arrays were used to measure the expression profiles of NF-κB in which 40 cycles were accomplished, 1 cycle for 10 min at 95°C and 40 cycles of 15 s at 95°C and 1 min at 60°C. Thereafter, Qiagen web-based data analysis was used to analyse the C_t values and then GraphPad Prism 4 was used to plot the graphs.