The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages

Figure 3

Determination of the apoptosis effect of lithium on Raw 264.7 macrophage cells using fluorescent microscopy. Cells were cultured at a density of 6 × 10 ⁵ cell/ml on coverslip in 6-well plates. Followed by treating cell with lithium 10 mM, NaCl 10 mM, actinomycin-D 0.02 mg/ml, and untreated cell were used as control for 24 hrs. Staining with Annexin-V and PI followed for 30 min at RT in the dark. Thereafter, cells were fixed and mounted on the slides and then measurement of fluorescence was accomplished by capturing pictures at 20x magnification with Nikon Ti-E inverted fluorescent microscope.