Impact of Vitamin C on Gene Expression Profile of Inflammatory and Anti-Inflammatory Cytokines in the Male Partners of Couples with Recurrent Pregnancy Loss

Table 1

Oligonucleotide primers.

Accession numbers	Gene	Primer sequence (5′-3′)	PCR product (bp)
NM_001382624.1	IL-10	F: GGTTGCCAAGCCTTGTCTGA; R: AGGGAGTTCACATGCGCCT	101
NM_001354990.2	IL-4	F: ACCTCGACTCGCCTACAAAG; R: TTCCTGTCGAGCCGTTTC	184
NM_000660.7	TGF-β	F: GTACCTGAACCCGTGTTGCT; R: GAACCCGTTGATGTCCACTT	288
NM_001371096.1	IL-6	F: GGTACATCCTCGACGGCATCT; R: GTGCCTCTTTGCTGCTTTCAC	81
NM_000594.4	TNF-⍺	F: CCCAGGCAGTCAGATCATCTT; R: AGCTGCCCCTCAGCTTGA	85
NM_000619.3	IFN-γ	F: AGCGGATAATGGAACTCTTTTCTT; R: AAGTTTGAAGTAAAAGGAGACAATTTGG	103
NM_001357943.2	GAPDH	F: GTATGACAACGAATTTGGCTACAG; R: GTCTCTCTCTTCCTCTTGTGCTCT	119

IL-10, interleukin 10; IL-4, interleukin 4, TGF-β, transforming growth factor-beta; IL-6, interleukin 6; TNF-α, tumor necrosis factor-alpha; IFN-γ, interferon-gamma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; F, forward; R, reverse.