Synthesis, Characterization, and Antimicrobial Activity of a Novel Trisazo Dye from 3-Amino-4H-thieno[3,4-c][1]benzopyran-4-one

Table 2

Minimum inhibitory concentrations (MICs) and minimum microbicidal concentrations (MMCs) (µg/ml) of compound 7 and its entire precursors against fungal and bacterial strains.

Microorganisms	Inhibition parameters	3	4	6	7	Reference
Gram-positive bacteria
Bacillus subtilis	MIC	64	16	64	8	2
	MBC	256	32	>256	32	2
S. aureus	MIC	64	16	32	2	0.5
	MBC	>256	32	64	8	0.5
Gram-negative bacteria
E. coli S2(1)	MIC	64	32	32	8	2
	MBC	256	64	>256	32	2
S. flexneri	MIC	128	32	128	16	8
	MBC	256	>256	>256	32	8
P. aeruginosa	MIC	128	16	128	8	4
	MBC	>256	32	>256	32	4
V. cholerae NB2	MIC	>256	256	256	64	32
	MBC	nd	>256	>256	128	32
V. cholerae SG24	MIC	>256	>256	>256	128	64
	MBC	nd	nd	nd	256	64
V. cholerae CO6	MIC	256	64	>256	32	8
	MBC	>256	128	nd	64	8
Yeasts
C. parapsilosis ATCC22019	MIC	64	4	32	4	2
	MFC	256	8	64	4	2
C. albicans ATCC9002	MIC	64	8	64	4	4
	MFC	>256	16	128	8	4
C. neoformans IP95026	MIC	32	4	8	4	2
	MFC	128	8	16	4	2

for bacteria and nystatin for yeasts; nd: not determined; compounds 1, 2, and 5 were not tested.