Table of Contents
International Journal of Molecular Imaging
Volume 2011 (2011), Article ID 405840, 9 pages
http://dx.doi.org/10.1155/2011/405840
Research Article

Comparison of the In Vivo Distribution of Four Different Annexin A5 Adducts in Rhesus Monkeys

1Imaging Research, Merck Research Laboratories, West Point, PA 19486, USA
2Department of Biochemistry, University Maastricht, 6200 NL Maastricht, The Netherlands
3Diabetes Exploratory Biomarker, Merck Research Laboratories, West Point, PA 19486, USA

Received 5 October 2010; Revised 19 January 2011; Accepted 23 February 2011

Academic Editor: Habib Zaidi

Copyright © 2011 Paul McQuade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Annexin A5 has been used for the detection of apoptotic cells, due to its ability to bind to phosphatidylserine (PS). Four different labeled Annexin A5 adducts were evaluated in rhesus monkey, with radiolabeling achieved via 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Of these adducts differing conjugation methods were employed which resulted in nonspecific radiolabeling (AxA5-I), or site-specific radiolabeling (AxA5-II). A nonbinding variant of Annexin A5 was also evaluated (AxA5-IINBV ), conjugation here was site specific. The fourth adduct examined had both specific and nonspecific conjugation techniques employed (AxA5-IImDOTA ). Blood clearance for each adduct was comparable, while appreciable uptake was observed in kidney, liver, and spleen. Significant differences in uptake of AxA5-I and AxA5-II were observed, as well as between AxA5-II and AxA5-IINBV . No difference between AxA5-II and AxA5-IImDOTA was observed, suggesting that conjugating DOTA nonspecifically did not affect the in vivo biodistribution of Annexin A5.