In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis
Figure 3
(a) Activation of QCASP3.2 by recombinant caspases. R.F.U.: relative fluorescent units. Cleavage by caspases 3 and 7 in the absence or presence of the caspase inhibitor Ac-Asp-Glu-Val-Asp-CHO (250 nM) is shown. No fluorescence was detected with caspases 1, 6, 8, and 11, thus these data are not presented. (b) Activation of QCASP3.2 by lysates of HME cells. Cells were either untreated (control) or treated with staurosporine 1 μM for 6 h, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH2F (100 μM). R.F.U.: relative fluorescent units. Data are mean ± SEM (). ** versus control and †† versus staurosporine alone by repeated measures ANOVA. (c) Colocalization of activated QCASP3.2-induced fluorescence (yellow) with caspase-3 IF (red) in HME cells. Cells were treated or not with staurosporine 1 μM for 6 hours, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH2F (100 μM). Nuclei are stained with Hoechst and appear blue. Orange staining is the colocalization of QCASP3.2 (yellow) with caspase-3 mAb (red; magnification x20).
(a) Activation of QCASP3.2 by recombinant caspases
(b) Activation of QCASP3.2 by cell lysates
(c) Colocalization of activated QCASP3.2 with caspase-3 in cultured cells
