Molecular Image Analysis: Quantitative Description and Classification of the Nuclear Lamina in Human Mesenchymal Stem Cells

Figure 2

Result of lamina image processing for cells at passage 4: (a,b) anisotropy is caused by spatial sampling and an anisotropic objective lens and (c) lamina segmentation. Top: x-y slice; Bottom: x-z slice. (a–c) image from a living cell, lamin A is visualized with a GFP fusion; (d) an image from a fixed cell, lamin A is detected with antibody staining.

(a) confocal image before image processing

(b) Resampling and blurring

(c) Edges of the lamina mask depicted in red and the convex hull in green. The overlap between lamina and convex hull is shown in yellow. Lines are dilated to improve visibility

(d) Example of lamina staining with an unusable segmentation. Lines are dilated to improve visibility