Table of Contents
International Journal of Molecular Imaging
Volume 2011 (2011), Article ID 893129, 12 pages
http://dx.doi.org/10.1155/2011/893129
Research Article

Longitudinal Evaluation of Fatty Acid Metabolism in Normal and Spontaneously Hypertensive Rat Hearts with Dynamic MicroSPECT Imaging

Department of Radiotracer Development & Imaging Technology, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA

Received 2 August 2010; Revised 15 September 2010; Accepted 16 September 2010

Academic Editor: Seigo Kinuya

Copyright © 2011 Bryan W. Reutter et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of this project is to develop radionuclide molecular imaging technologies using a clinical pinhole SPECT/CT scanner to quantify changes in cardiac metabolism using the spontaneously hypertensive rat (SHR) as a model of hypertensive-related pathophysiology. This paper quantitatively compares fatty acid metabolism in hearts of SHR and Wistar-Kyoto normal rats as a function of age and thereby tracks physiological changes associated with the onset and progression of heart failure in the SHR model. The fatty acid analog, 123I-labeled BMIPP, was used in longitudinal metabolic pinhole SPECT imaging studies performed every seven months for 21 months. The uniqueness of this project is the development of techniques for estimating the blood input function from projection data acquired by a slowly rotating camera that is imaging fast circulation and the quantification of the kinetics of 123I-BMIPP by fitting compartmental models to the blood and tissue time-activity curves.