Research Article

64Cu-NODAGA-c(RGDyK) Is a Promising New Angiogenesis PET Tracer: Correlation between Tumor Uptake and Integrin Expression in Human Neuroendocrine Tumor Xenografts

Table 2

Final QPCR designs for human and mice genes of interest and for housekeeping genes.

GeneNM noForward primer, 5′-3′Reverse primer, 5′-3′5′-FlourophoreTaqMan probe, 5′-3′3′-QuencherAmplicon length (bp)

Humane

h-ITGAVNM_002210GGGTCAAGATCAGTGAGAAATCTTTACATTCCTGTAACATCATGCTATTGCTAGFAMAGGAACCTGGACCCCTTACCCCAACTTTBHQ-1139
h-ITGB3NM_000212CTCCTGTCCCTCATCCATAGCCAGCCAAGAGGTAGAAGGTAAATACCY5ACAGCACACCAAGGCACAGGGCBHQ-2103
h-VEGFANM_001025366GTGTGAGTGGTTGACCTTCCTCCCGTATATAAAACACTTTCTCTTTTCTCTGHEXCCTGGTCCTTCCCTTCCCTTCCCGABHQ-1125

Mice

m-ITGAVNM_008402ACGTTACATAGCATAGTACCTCTTCTACTGATGGTCTAAATTTGAACTGCFAMTGCCCTAACGTAACGTCGAAGAGAGAAACCBHQ-1121
m-ITGB3NM_016780TCAACATCCTAAGATGCCGAGAGCTGCTTGTTCTACTACTGGTCAACCY5ACGGTATCAAGCCTGCGGTCCAGATTCBHQ-2104
m-VEGFANM_001025250GGGACCCCTTCGTCCTCTCGTCTCCTGGGGACAGAATTAGTGHEXATCACACAAGGTCCTCCTGGGCTGTBHQ-1100

Housekeeping genes

h-HPRTNM_000194AAGCCTAAGATGAGAGTTCAAGCTCTACTAAGCAGATGGCFAMAACATCTGGAGTCCTATTGACATCGCBHQ-1108
h-UBCNM_021009CTGGAAGATGGTCGTACCGTCAGGGTCTTCACGAAGHEXCACCTCTGAGACGGAGCACCBHQ-1107
h-RPLPNM_001002TGTGCAGCTGATCAAGACAGCACTTCAGGGTTGTAGCY5CTGCCATTGTCGAACACCTGCBHQ-2141

Table 2 shows the primers and probe sequences, the flourophores and quenchers used on the TaqMan probes and the amplicon length of the product for each gene.