The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts
Figure 3
Effect of acute hypoxia and reoxygenation on BLS. U87-Luc cell pellets (a) or monolayers (b) were exposed to Oxyrase for 5 min to induce acute hypoxia before addition of luciferin and BLI. After Oxyrase addition and BLI, monolayers were reoxygenated by bubbling 1 mL of air through the media in each well; subsequent BLI is shown in (b) and results for the 200 mU/mL Oxyrase treatment are quantified in (c). Monolayers and cell pellets treated with Oxyrase that had been heat inactivated (boiling for 10 min) were assessed by BLI (d).
