Research Article

The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts

Figure 6

Effect of acute ischemia and reperfusion on in vivo BLS. (a) BLS was collected for 1 min in a nude mouse bearing bilateral U87-Luc subcutaneous thigh xenografts 5 min after injection of D-luciferin. The mouse was treated with a right thigh tourniquet proximal to the tumor. BLI was performed 2 min after tourniquet application and then 2 min after tourniquet removal. (b) BLS was collected at 1 min intervals for 60 min in nude mice bearing bilateral U87-Luc subcutaneous thigh xenografts. At 30 min, nicotinamide was administered via IP injection.
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