In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation
Figure 2
In vivo fluorescence intensity analysis over time and whole body scan biodistribution of 3 × 106 mAT-MASCeGFP labelled with DiD in a healthy animal model after i.m. injection in the left tibialis anterior muscle (a). In vivo fluorescence intensity analysis of contralateral (and not injected) leg. The scans were optimized in this region to visualize changes in terms of fluorescence intensity. The peak is reached 48 hours after the injection of the labelled cells (b). (c) In vivo lifetime analysis and comparison between the control (A), the contralateral leg 48 hours after mAT-MASCeGFP-DiD i.m. injection (B), and the injected muscle (C). (B) and (C) have comparable trends and comparable value (see Table 2). (c) In vitro lifetime analysis of mAT-MASCeGFP-DiD (D) and free DiD (E): the different trend of the curves indicates the possibility to discriminate by a lifetime analysis between the labelled cells and the pure dye (for the values see Table 2).
