Research Article

In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation

Figure 3

(a) Ex vivo fluorescence intensity analysis 48 hours after i.m. mAT-MASC-DiD injection. Mice were sacrificed, and organs were excided and analyzed by Optix. The fluorescence intensity map is shown. (b) Applying a lifetime gate (1.3–1.8 ns, corresponding to the lifetime range of DiD) to the initial intensity images yielded a map of mAT-MASCeGFP-DiD biodistribution and eliminated background signal. (c) The graph representing lifetime trends confirms the comparability between the signals recorded from the different organs ex vivo, which are represented by the same slope of the curves. The colors, respectively, represent yellow, liver; green, heart; red, brain; white, lung; grey, diaphragm; blue, spleen; pink, contralateral muscle.
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