In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation

Table 1

Cell and tissue staining protocols.

Primary antibody				Secondary antibody
Antigen	Producer	Antigen retrieval	Dilution	Fluorochrome	Producer	Dilution
OCT4	ABCAM	TRITONX-100 0.1%	1 : 200	A488	Molecular probes	1 : 400
NANOG	ABCAM	TRITONX-100 0.1%	1 : 400	A488	Molecular probes	1 : 400
SOX2	ABCAM	TRITONX-100 0.1%	1 : 200	A488	Molecular probes	1 : 400
eGFP	ABCAM	TRITONX-100 0.1%	1 : 80	A488; A555	Molecular probes	1 : 400; 1 : 600
MCM5	ABCAM; ABCAM	TRITONX-100 0.1%	1 : 80; 1 : 100	A555; A488	Molecular probes	1 : 600; 1 : 400
eGFP; MMP2	ABCAM; ABCAM	TRITONX-100 0.1%	1 : 80; 1 : 80	A488; A555	Molecular probes	1 : 400; 1 : 600
eGFP; ASA	ABCAM; Sigma	TRITONX-100 0.1%	1 : 80; 1 : 100	A488; TRITC [IgM]	Molecular probes; Jackson  ImmunoResearch	1 : 400; 1 : 100
eGFP; CD146	ABCAM; ABCAM	TRITONX-100 0.1%	1 : 80; 1 : 20	A488; A555	Molecular probes	1 : 400; 1 : 600
eGFP; SMA	ABCAM; DAKO	TRITONX-100 0.1%	1 : 80; 1 : 40	A555; A488	Molecular probes	1 : 400; 1 : 600
Laminin; ASA	DAKO; Sigma	TRITONX-100 0.1%	1 : 50; 1 : 100	A488; Cy5 [IgM]; A555	Molecular probes; Jackson ImmunoResearch	1 : 400; 1 : 100; 1 : 600