Research Article | Open Access
Shingo Chihara, Mary K. Hayden, Eileen Minogue-Corbett, Kamaljit Singh, "Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar", International Journal of Microbiology, vol. 2009, Article ID 636502, 3 pages, 2009. https://doi.org/10.1155/2009/636502
Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar
The ability to rapidly differentiate coagulase-negative staphylococcus (CoNS) from Staphylococcus aureus and to determine methicillin resistance is important as it affects the decision to treat empiric antibiotic selection. The objective of this study was to evaluate CHROMagar S. aureus and CHROMagar MRSA (Becton Dickinson) for rapid identification of Staphylococcus spp. directly from blood cultures. Consecutive blood culture bottles (BacT Alert 3D SA and SN, bioMérieux) growing gram-positive cocci in clusters were evaluated. An aliquot was plated onto CHROMagar MRSA (C-MRSA) and CHROMagar S. aureus (C-SA) plates, which were read at 12 to 16 hours. C-SA correctly identified 147/147 S. aureus (100% sensitivity); 2 CoNS were misidentified as S. aureus (98% specificity). C-MRSA correctly identified 74/77 MRSA (96% sensitivity). None of the MSSA isolates grew on C-MRSA (100% specificity). In conclusion, CHROMagar is a rapid and sensitive method to distinguish MRSA, MSSA, and coagulase-negative Staphylococcus and may decrease time of reporting positive results.
Staphylococcus species are the most commonly isolated bacteria from blood cultures [1–3]. The ability to rapidly differentiate coagulase-negative staphylococcus (CoNS) from S. aureus is important as CoNS is a common skin contaminant whereas S. aureus bacteremia is associated with significant morbidity and mortality [2, 3]. In addition, the increased prevalence of methicillin resistance in Staphylococcus spp. has led to the widespread use of vancomycin as empiric therapy, which may provide selective pressure for the spread of vancomycin-resistant enterococcus and the emergence of vancomycin-intermediate S. aureus (VISA) and vancomycin-resistant S. aureus (VRSA) . The ability to rapidly differentiate CoNS from S. aureus and to determine methicillin susceptibility, particularly from blood cultures, is clearly desirable as it would allow more directed antibiotic therapy and limit the unnecessary use of vancomycin.
The objective of this study was to evaluate CHROMagar S. aureus (Becton Dickinson, Sparks, Md, USA) and CHROMagar MRSA (Becton Dickinson, Sparks, Md, USA) for rapid identification and susceptibility testing of Staphylococcus spp. directly from blood culture broth media.
2. Materials and Methods
The study was performed in the microbiology laboratory at Rush University Medical Center in Chicago (Ill, USA) from November 2006 to August 2007. Consecutive blood culture bottles (BacT/Alert 3D SE and SN bottles, bioMérieux, Durham, NC, USA) growing gram-positive cocci in clusters were evaluated. Only one set of blood cultures from each patient was included. A 0.05 mL aliquot of blood broth was plated onto CHROMagar MRSA (C-MRSA) and CHROMagar S. aureus (C-SA) plates that were divided into 4 quadrants to allow each plate to be used for 4 patient isolates. Plates were incubated at 35°C in ambient air. Growth on plates was evaluated after 12 to 16 hours of incubation and again after the manufacturer's recommended incubation time of 24 hours. Mauve colonies on C-SA plate were classified as S. aureus. Mauve colonies on C-MRSA were classified as MRSA. CoNS produced white colonies.
Results were compared to conventional microbiologic methods for identifying Staphylococcus spp., that is, gram stain, catalase reaction, and tube or slide coagulase testing (BactiStaph, Remel Inc. Lenexa, Kan, USA). Discrepant identification results were resolved by an automated identification method (Microscan Walkaway, Dade Behring Inc., West Sacramento, Calif, USA). Susceptibility testing was done by using the cefoxitin disk test according to CLSI guidelines  Methicillin resistance in S. aureus was confirmed by the detection of PBP2a using the MRSA screen slide agglutination test (Denka Seiken Co, LTD, Tokyo, Japan).
Two hundred and sixty three unique blood culture broths growing gram-positive cocci in clusters were studied. Five bottles that yielded Micrococcus spp. were excluded from further analysis; none of these produced mauve colonies on C-MRSA or C-SA plates. There were 147 S. aureus and 111 CoNS identified by conventional microbiologic methods. C-SA correctly identified 147/147 S. aureus after 16 hours of incubation (100% sensitivity). There were 2 CoNS isolates that produced faint mauve colonies on C-SA; these were confirmed BactiStaph negative, and were identified as Staphylococcus hominis and Staphylococcus haemolyticus. Therefore, the specificity of C-SA for identification of S. aureus was 98%. The positive predictive value of the C-SA plates for identification of S. aureus was 99% (147/149) while the negative predictive value was 100% (109/109).
C-MRSA correctly identified 74/77 MRSA (96% sensitivity) after 12 to 16 hours incubation. None of the MSSA or CoNS isolates grew on C-MRSA (100% specificity). Both C-SA and C-MRSA plates were read again after 24 hours incubation; the results were completely concordant with those obtained after 12 to 16 hours incubation (data not shown). The positive predictive value of the C-MRSA plates for identification of MRSA was 100% (74/74) while the negative predictive value was 98% (181/184).
Our study shows that the direct subculture of positive blood culture broths onto C-MRSA and C-SA plates allows for the rapid identification of S. aureus and determination of methicillin susceptibility. It is important to distinguish among MSSA, MRSA, and CoNS as management changes depending on the species isolated. It is a common practice among clinicians to initiate empiric vancomycin therapy when informed of a positive blood culture growing gram-positive cocci in clusters. The use of a beta-lactam antibiotic is preferred therapy if MSSA is isolated but current methods of susceptibility testing using oxacillin or cefoxitin disks require cultures to be held for 24 hours before susceptibility results can be reported. Hence, a decision to continue, stop, or change therapy is generally not possible in the first 24 to 48 hours of culture incubation using conventional microbiology methods. In addition, it is often recommended to place patients with MRSA infection on contact isolation as soon as possible; hence the rapid identification of MRSA is also an important infection control consideration.
CHROMagar S. aureus is a selective and differential medium developed to isolate and identify S. aureus. It has been used to identify S. aureus in clinical specimens such as respiratory samples and wound swabs [6, 7]. CHROMagar MRSA is a selective and differential medium developed to detect MRSA in nasopharyngeal specimens and thereby ascertain MRSA carriage . However, there are few data on the use of these plates as primary media for subcultures of positive blood culture bottles. In a study by Pape et al., only C-MRSA was utilized and the plates were read at 18 to 24 hours. This study showed that the medium was highly sensitive (97.6%) and specific (99.9%) for identifying MRSA from blood culture broths . In a similar study by Colakoglu et al., MRSA ID chromogenic medium (Becton Dickinson, Sparks, Md, USA) was used to identify MRSA directly from blood culture bottles, wound swabs, and abscesses. For blood cultures, the sensitivity was 97.8% and the specificity was 99.7% at 24 hours incubation . We elected to use both CHROMAgar C-SA and C-MRSA as we feel that it is important to be able to rapidly identify both MSSA and MRSA bacteremia. We read C-SA and C-MRSA plates at 12 to 16 hours rather than at 18 to 24 hours of incubation as we surmised that there are significantly higher organism loads in blood culture broths compared to nasal swab specimens and most positive blood cultures are pure cultures compared to the mixed flora seen from nasal swabs. There was no discrepancy between the 12- to 16-hour and 24-hour readings.
While there are alternative methods to rapidly identify Staphylococcus spp., such as real-time PCR, they are more expensive, require specialized equipment and sometimes batch testing of specimens . Direct tube coagulase test is a simple, inexpensive, and rapid method to test for coagulase-positive and coagulase-negative Staphylococcal spp. in blood culture broths, but the sensitivity of the test has been reported as low as 65% [12, 13]. In our study, the direct tube coagulase test had a high specificity (100%), but a sensitivity of only 71% (data not shown).
In the current study, there were 2 false-positive C-SA cultures that grew CoNS and these were identified as S. hominis and S. haemolyticus. Previous reports have suggested that other coagulase-negative Staphylococcus spp. including S. cohnii, S. intermedius, and S. schleiferi, may yield a faint mauve color on C-SA that with experience can be distinguished from the deep mauve color of S. aureus . We suggest performing a gram-stain and coagulase test when there is a question as to the final identification of the organism isolated.
While none of the MSSA isolates produced mauve colonies on C-MRSA (negative predictive value 100%), there were three false negative MRSA isolates which did not give mauve colonies on C-MRSA (positive predictive value 98%). This suggests that while a positive result by C-MRSA can be reported as MRSA, a negative result should only be reported as S. aureus with susceptibility to be followed by conventional testing.
In conclusion, CHROMagar is a rapid and sensitive method to distinguish MRSA, MSSA, and CoNS directly from blood culture bottles. It is an easy method that can fit into the routine workflow of most clinical microbiology laboratories and that does not require any new or expensive equipment. Use of C-SA and C-MRSA may facilitate a decreased time for reporting positive results for MSSA and MRSA bacteremia.
These data were presented in part at the 47th Interscience Conference on Antimicrobial Agents and Chemotherapy, September 17–20, 2007, in Chicago, Ill, USA.
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Copyright © 2009 Shingo Chihara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.