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International Journal of Microbiology
Volume 2009, Article ID 917623, 13 pages
Research Article

Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue City, Shimane 690-0122, Japan

Received 4 November 2008; Revised 19 December 2008; Accepted 4 February 2009

Academic Editor: Todd R. Callaway

Copyright © 2009 Hiroshi Fukushima et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A set of four duplex SYBR Green I PCR (SG-PCR) assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. The causative pathogens were detected in almost all cases in 2 hours or less. The first run was for the detection of 8 main foodborne pathogens in 5 stool specimens within 2 hours and the second run was for the detection of other unusual suspect pathogens within a further 45 minutes. After 2 to 4 days, the causative agents were isolated and identified. The results proved that for comprehensive and rapid molecular diagnosis in foodborne outbreaks, Duplex SG-PCR assay is not only very useful, but is also economically viable for one-step differentiation of causative pathogens in fecal specimens obtained from symptomatic patients. This then allows for effective diagnosis and management of foodborne outbreaks.