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International Journal of Microbiology
Volume 2010, Article ID 150464, 8 pages
Research Article

Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)

1Service de Bactériologie, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, France
2Service d'Epidémiologie, Hygiène Hospitalière et Santé Publique, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, France
3Unité de Virologie clinique et fondamentale, Faculté de Médecine et de Pharmacie, 3 rue des Louvels, 80036 Amiens, Cedex, France
4Service de Réanimation Néphrologique, CHU Sud, avenue René Laënnec, 80054 Amiens Cedex 1, France
5Service de Pathologie Infectieuse, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, France

Received 30 July 2010; Revised 15 September 2010; Accepted 17 September 2010

Academic Editor: William M. Shafer

Copyright © 2010 M. Biendo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We studied 138 glycopeptide-resistant enterococci (GRE) strains, consisting of 131 glycopeptide-resistant Enterococcus faecium (GREfm) and 7 glycopeptide-resistant Enterococcus faecalis (GREfs). The GREfm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the GREfs strains were only resistant to vancomycin and teicoplanin. The van A gene was the only glycopeptide determinant present in all GRE isolates investigated. Genes coding for Hyl and Hyl+ Esp were detected in 39 (29.8%) and 92 (70.2%) of the 131 GREfm isolates, respectively. Three of the 7 GREfs were positive for gelE+asa 1 genes, 3 for gel E gene, and 1 for asa 1 gene. The genetic relationship between the 138 GRE was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). GREfm isolates were clustered in a single genogroup (pulsotype A), and GREfs were clustered in six genogroups (pulsotypes B-G). Among the isolates investigated by MLST, only 18 PCR products were sequenced (12 E. faecium and 6 E. faecalis), and 9 sequence types (STs) were identified.