Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)

Table 3

Oligonucleotide primers used to amplify virulence factor genes.

Gene	Virulence factor	Primer name	Oligonucleotide sequence (5^′ to 3^′)	Product size (bp)
asa1	Aggregation substance, encoded by the plasmid asa1	ASA 11	GCA-CGC-TAT-TAC- GAA -CTA-TGA	375
		ASA 12	TAA-GAA-AGA-ACA-TCA-CCA-CGA
gelE	Gelatinase, encoded by the chromosomal gelE	GEL 11	TAT-GAC-AAT-GCT-TTT-TGG-GAT	213
		GEL 12	AGA-TGC-ACC-CGA-AAT-AAT-ATA
cylA	Cytolysin, encoded by the plasmid cylA	CYT I	ACT-CGG-GGA-TTG-ATA-GGC	688
		CYT II	GCT-GCT-AAA-GCT-GCG-CTT
esp	Enterococcal surface protein, encoded by the chromosomal esp	ESP 14F	AGA-TTT-CAT-CTT-TGA-TTC-TTG-G	510
		ESP 12R	AAT-TGA-TTC-TTT-AGC-ATC-TGG
hyl	Hyaluronidase, encoded by the chromosomal hyl	HYL n1	ACA-GAA-GAG-CTG-CAG-GAA-ATG	276
		HYL n2	GAC-TGA-CGT-CCA-AGT-TTC-CAA