Table of Contents Author Guidelines Submit a Manuscript
International Journal of Microbiology
Volume 2010 (2010), Article ID 430873, 9 pages
http://dx.doi.org/10.1155/2010/430873
Research Article

Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria

1Department of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07 Uppsala, Sweden
2Department of Chemistry, Swedish University of Agricultural Sciences, P.O. Box 7015, 750 07 Uppsala, Sweden

Received 19 May 2010; Revised 24 November 2010; Accepted 28 November 2010

Academic Editor: Isabel Sá-Correia

Copyright © 2010 Helena Lind et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dairy propionibacteria are widely used in starter cultures for Swiss type cheese. These bacteria can ferment glucose, lactic acid, and glycerol into propionic acid, acetic acid, and carbon dioxide. This research examined the antifungal effect of dairy propionibacteria when glycerol was used as carbon source for bacterial growth. Five type strains of propionibacteria were tested against the yeast Rhodotorula mucilaginosa and the molds Penicillium commune and Penicillium roqueforti. The conversion of 13C glycerol by Propionibacterium jensenii was followed with nuclear magnetic resonance. In a dual culture assay, the degree of inhibition of the molds was strongly enhanced by an increase in glycerol concentrations, while the yeast was less affected. In broth cultures, decreased pH in glycerol medium was probably responsible for the complete inhibition of the indicator fungi. NMR spectra of the glycerol conversion confirmed that propionic acid was the dominant metabolite. Based on the results obtained, the increased antifungal effect seen by glycerol addition to cultures of propionibacteria is due to the production of propionic acid and pH reduction of the medium.