Expression and Localization of an Hsp70 Protein in the Microsporidian Encephalitozoon cuniculi
Figure 2
Immuno-TEM of E. cuniculi infected rabbit kidney cells. The infected cells were prepared according to the protocol described. Ultrathin Lowicryl resin sections were cut and reacted with C1 Hsp70 specific antibodies (1 : 10) and a secondary antibody conjugated to 15-nm gold particles (1 : 200). The developmental stages include immature meronts and sporonts (the lower and upper spores in (a), resp.) and a mature spore (b). Abbreviations are as follows: PTC, polar tube coil; N, nucleus; PV, posterior vacuole; AD, anchoring disk. Each bar indicates a 500-nm scale.
