International Journal of Microbiology

Research Article

Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis

Table 2

659 bacterial strains assayed by real-time PCR.
BacterialPresence ofNumber of strainPCR positive result with each primer se
strainPCR targetControleaeJMJMLTSTaaggEASdaaipainvyaPSPAAceuAHomptdhtrhGAPLm-FecesSGyersyersH2
gen strainS1S2RTDHAdAGGBEH1WhlymB
Escherichia coli—STECeae, stx1, stx2 SE0200720202020−−−−−−−−−−−−−−−−−−−−−−−
eae, stx1151515−−−−−−−−−−−−−−−−−−−−−−−−
eae, stx277−7−−−−−−−−−−−−−−−−−−−−−−−
stx1, stx22−22−−−−−−−−−−−−−−−−−−−−−−−
stx21−−1−−−−−−−−−−−−−−−−−−−−−−−
E. coli—EPECeaeEC273 33−−−−−−−−−−−−−−−−−−−−−−−−−
eae, astA55−−−−−5−−−−−−−−−−−−−−−−−−−
E. coli—ETECLT3−−−3−−−−−−−−−−−−−−−−−−−−−−
ST9−−−−8−−−−−−−−−−−−−−−−−−−−−
astA, LT1−−−1−−1−−−−−−−−−−−−−−−−−−−
astA, LT,STEC351 2−−−22−2−−−−−−−−−−−−−−−−−−−
astA, ST7−−−−3−7−−−−−−−−−−−−−−−−−−−
E. coli—ETECastA, aggREC413 8−−−−−88−−−−−−−−−−−−−−−−−−−
aggR26−−−−−26−−−−−−−−−−−−−−−−−−−−
astA30−−−−−−30−−−−−−−−−−−−−−−−−−−
E. coli—DAECdaaD, astA2−−−−−−22−−−−−−−−−−−−−−−−−−
daaDKI221 2−−−−−−−2−−−−−−−−−−−−−−−−−−
E. coli—EIECipaHEA3 5−−−−−−−−5−−−−−−−−−−−−−−−−−
Shigella spp.ipaHI0003138−−−−−−−−38−−−−−−−−−−−−−−−−−
Salmonella spp.invASal233931−−−−−−−−−31−−−−−−−−−−−−−−−−
Yersinia enterocoliticayadAPa24128−−−−−−−−−−28−−−−−−−−−−−−−−−
Y. pseudotuberculosisyadASP98827−−−−−−−−−−27−−−−−−−−−−−−−−−
Plesiomonas shigelloidesgyrBNIID12 4−−−−−−−−−−−4−−−−−−−−−−−−−−
Providencia alcalifaciensgyrBNIID12 8−−−−−−−−−−−−8−−−−−−−−−−−−−
Campylobacter jejunispecific geneSC0143−−−−−−−−−−−−−43−−−−−−−−−−−−
Campylobacter coliceuESC00913−−−−−−−−−−−−−−13−−−−−−−−−−−
Aeromonas hydrophilaahh1AT CC796645−−−−−−−−−−−−−−−45−−−−−−−−−−
Vibrio choleraeOmpWAT CC1403517−−−−−−−−−−−−−−−−17−−−−−−−−−
Vibrio parahaemolyticustdhSVP0248−−−−−−−−−−−−−−−−−48−−−−−−−−
tdh, trh2−−−−−−−−−−−−−−−−−22−−−−−−−
trhNIIDk 35−−−−−−−−−−−−−−−−−−35−−−−−−−
Clostridium perfringenscpeH 41−−−−−−−−−−−−−−−−−−−41−−−−−−
Listeria monocytogeneshlyScott A46−−−−−−−−−−−−−−−−−−−−46−−−−−
Staphylococcus aureusFemBSS0535−−−−−−−−−−−−−−−−−−−−−35−−−−
Emetic Bacillus cereus ces, nheBNo.12 24−−−−−−−−−−−−−−−−−−−−−−2424−−
Enterotoxgenic B. cereus nheBNo. 25−−−−−−−−−−−−−−−−−−−−−−−25−−
Yersinia ruckeri16S rRNAJCM151101−−−−−−−−−−−−−−−−−−−−−−−−11
resence of PCR target gene was determined by another conventional PCR primer sets before this test.
train kindly donated by J. Yatsuyanagi, Akita Prefectural Institute of Public Health (Akita, Japan) . Ito, National Institute of Infectious Disease (Tokyo, Japan)., . Sugiyama, Shizuoka Prefectural Institute of Public Health (Shizuoka, Japan), . Arakawa, National Institute of Infectious Disease (Tokyo, Japan), . Kaneko, Tokyo Metropolitan Institute of Public Health (Tokyo, Japan), . Ueda, Kagawa Nutrition University (Saitama, Japan) umber positive result; − negative result. See Table 2 for primer sets.