Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis

Table 4

The relationship between PCR detection and CFU in 15 foodborne outbreak cases by viable cell counting.

		Multiplex SG-PCR negative samples						Multiplex SG-PCR positive samples
Case	Causative foodborne pathogens	Number of samples						Number of samples
		Total	log 10 cfu/g by viable cell counting					Total	Log 10 cfu/g by viable cell counting
			0	2	3	4	5		0	2	3	4	5	6	7	8
3	C. perfringens	2	2					5					1	3		1
6	C. jejuni	3	2		1			4				1	3
8	C. perfringens	4	2		1	1		2				1	1
9	C. jejuni	3	3					2				2
10	EHEC O26	3	2		1			4			1			1	1	1
11	astA-positive E. coli	0						7						1		6
13	S. aureus	4	3			1		1				1
14	C. perfringens	0						7					1	3	3
17	B. cereus	3	3					1				1
18	C. jejuni	1					1	5					1		2	2
20	C. jejuni	3	3					4			1		3
19	P. shigelloides	3	3					2					2
	astA-positive E. coli	4	4					1				1
21	C. jejuni	3	3					4					3	1
26	S. enterica serovar Enteritidis	2				2		0
27	EHEC O157	5	5					2		1	1
	Total	43	35	0	3	4	1	51	0	1	3	7	15	9	6	10