International Journal of Microbiology

Review Article

The S-Layer Glycome—Adding to the Sugar Coat of Bacteria

Table 1

Overview on S-layer glycoprotein-covered bacteria.


Organism	Gene designation/Accession number	Lattice /Lattice dimensions (nm)/Molecular mass (kD)	Biochemical evidence	Reference

Aneurinibacillus thermoaerophilus, strain L420-	satA/AY395578 slg cluster/AY442352	S/10.0/116	O-glycan structure Linkage region	[3, 18, 21]
Aneurinibacillus thermoaerophilus DSM 10155/G⁺	satB/AY395579	S/10.0/153	O-glycan structure Linkage region	[18, 22, 23]
Bacillus smithii, several strains		O /~11.2 and 10.3/132–138	Detection of glycoses	[24]
Bacillus sp., several strains		O,S,H/5–23/66–255	SDS-PA, staining	[25]
Bacillus thuringiensis, several strains			SDS-PA, staining	[26]
Campylobacter rectus, strain ATCC 33238	slp/AB001876	H//150	Western blot	[27]
Deinococcus radiodurans, strain Sark	HPI gene/M17895	H/18/107	SDS-PA, staining, Monosaccharide analysis	[28, 29]
Deinococcus radiodurans, strain R1	hpi/DR2508, slpA/DR1185	H//99(37)	SDS-PA, staining	[30]
Geobacillus stearothermophilus, strain ATCC 12	sbsC/AF055578 dTDP-L-Rha operon/AY278519	O/9.5 and 5.2/122	Detection of L-rhamnose	[31, 32]
Geobacillus stearothermophilus ATCC 12980 variant	sbsD/AF228338	O//–175	SDS-PA, staining, Monosaccharide analysis	[33]
Geobacillus stearothermophilus, strain NRS 2004/3a	sgs /AF328862 slg cluster/AF328862	O/11.6 and 9.4/ –170	O-glycan structure Linkage region Biosynthesis	[32, 34, 35]
Geobacillus stearothermophilus, strain L32-65	sgsF/DQ414249 dTDP-L-Rha operon/AY278518	O/11.9 and 8.6/96	Detection of L-rhamnose	[32]
Geobacillus tepidamans, strain GS5-97^T	sgtA/AY883421 slg cluster/ AY883421	O/11.2 and 7.9/106–166	O-glycan structure Linkage region	[36–38]
Hyphomonas jannaschiana, strain ATCC 33884		+ 116	SDS- PA, staining	[39]
Lactobacillus buchneri, strain 41021/251		O/6.1 and 5.4/53	O-glycan structureLinkage region	[40]
Lactobacillus helveticus, strain ATCC 12046		O/9.6 and 4.5/43.5	SDS-PA, staining	[41]
Lactobacillus kefir JCM 5818 and several other strains			SDS- PA, staining Mass spectrometry	[42, 43]
Methylomicrobium alcaliphilum		H/36/10–45	SDS- PA Monosaccharide analysis	[44]
Paenibacillus alvei, strain CCM	spaA/FJ751775	O/10.0 and 7.9/240, 160,	O-glycan structure Linkage region Biosynthesis	[45–48]
Parabacteroides distasonis	DgpA, Dgp	, 90	SDS-PA, staining Monosaccharide analysis	[20]
Sulfobacillus thermosulfidooxidans			Monosaccharide analysis	[49]
Synechococcus sp., strain WH8102	swmA/AF056046	O/	SDS- PA, staining	[50, 51]
Tannerella forsythia, strain ATCC 43037	tfsA + tfsB/AY423857	+ 210	SDS- PA, staining	[19, 52, 53]
Thermoanaerobacter kivui, strain DSM 2030	slp/M31069	H/19 /90	SDS- PA, Deglycosylation	[54]
Thermoanaerobacter thermohydrosulfuricus, strain L111-69	surA/AJ401026 sttA/FJ656210	H/13.9/130	O-glycan structure Linkage region	[55, 56]
Thermoanaerobacter thermohydrosulfuricus, strain S102-70		H/16.3/94	O-glycan structure Linkage region	[57, 58]
Thermoanaerobacter thermohydrosulfuricus, strains L77-66 and L92-71		H/14.3/, 90–200	O-glycan structure Deglycosylation	[59]
Thermoanaerobacterium thermosaccharolyticum, strain E207-71	slg cluster/AY422724	S /12/–210	O-glycan structure	[18, 60]
Thermoanaerobacterium thermosaccharolyticum, strain D120-70		S/11/80^d–170	O-glycan structure Linkage region	[61]
Thermoanaerobacterium thermosulfurigenes, strain EM1		–190	SDS- PA, staining Monosaccharide analysis	[62]

Abbreviations: O, oblique (p1, p2); S, square (p4); H, hexagonal (p3, p6); SDS-PAGE, sodiumdodecylsulfate polysaccharide gel electrophoresis; PAS, periodic acid-Schiff staining; deglycosylated protein.