International Journal of Microbiology

Research Article

Bacillus Strains Most Closely Related to Bacillus nealsonii Are Not Effectively Circumscribed within the Taxonomic Species Definition

Table 1

Presumptive signature sequences (PSS) conserved in 16S rRNA gene at nt positions 71–92a,b (PSS1) and 183–223a (PSS2) in respective V1 and V2 hypervariable regions of Bacillus nealsonii-related strains. aNumbering based on rrnE of Bacillus subtilis subsp subtilis strain (NC_000964.3); bgap at nt position 80 in PSS1 sequence alignments to rrnE is reflected in the numbering.
PSS typeBacillus Strain no.GenBank Accession no.Nucleotides of PSS
PSS1AATCC AY724690G A C T T T A A AbA G C T T G C T T T T A
B. circulans
N3 B. circulans AB215100G A C T T T A A AA G C T T G C T T T T A
PSS1BDSM EU656111G A C T T A A A AA G C T T G C T T T T T
B. nealsonii
CBD 118DQ374636G A C T T A A A AA G C T T G C T T T T T
OSS 25EU683686G A C T T A A A AA G C T T G C T T T T T
P307FJ943260G A C T T A A A AA G C T T G C T T T T T
P308FJ554672G A C T T A A A AA G C T T G C T T T T T
C4T1F3B3FJ943258G A C T T A A A AA G C T T G C T T T T T
IAFILS6FJ943259G A C T T A A A AA G C T T G C T T T T T
AD5AFJ943256G A C T T A A A AA G C T T G C T T T T T
U4AFJ943261G A C T T A A A AA G C T T G C T T T T T
ADP4IIFJ943257G A C T T A A A AA G C T T G C T T T T T
PSS2aCBD 118DQ374636CCTTTTCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
OSS 25EU683686CCTTTTCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
CT18EU660368CCTTTTCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
Q2CJ3GU471201CCTTTTCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
PSS2bP307FJ943260CCTTTCCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
P308FJ554672CCTTTCCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
C4T1F3B3FJ943258CCTTTCCTACTCATGTAGGAAAG—CTGAAAGACGGTTTA—CGC
PSS2cIAFILS6FJ943259CCTTTTCTACTCATGTAGAAAAG—CTGAAAGACGGTTTA—CGC
AD5AFJ943256CCTTTTCTACTCATGTAGAAAAG—CTGAAAGACGGTTTA—CGC
U4AFJ943261CCTTTTCTACTCATGTAGAAAAG—CTGAAAGACGGTTTA—CGC
ADP4IIFJ943257CCTTTTCTACTCATGTAGAAAAG—CTGAAAGACGGTTTA—CGC
PSS2  B. nealsonii DSM EU656111CCTTTTCTACTCATGTAGAGAAGTCTGAAAGACGGCATCTCGC
PSS2  B. circulans ATCC AY724690CCTTTTCCTCTCATGAGGAAAAG—CTGAAAGACGGTTTA—CGC