Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates
Table 3
Combined-PATS profiles for the 25 bovine O157 isolates.
PCR amplification and restriction digestion patterns of amplicons obtained using 15 PATS—4 virulence gene primer pairsb
PATS typea
Polymorphic XbaI sites
Polymorphic AvrII sites
Virulence genes
Isolatesc
IK8
IK19
IK25
IK114
IK118
IK123
IKB3
IKB5
—
IKNR3
IKNR7
IKNR10
IKNR12
IKNR16
IKNR27
IKNR33
—
stx 1
stx 2
eae
hlyA
1 (previous 19)d
2
2
0
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
168, 908, 928
2 (previous 2)
0
2
0
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
977
3 (previous 18)
2
2
0
2
2
2
0
2
2
2
2
2
2
2
2
1
1
1
1
5, 817, 1015, 1492
4 (previous 16)
2
2
0
2
2
2
0
2
2
2
2
0
2
2
2
1
1
1
1
757, 806
5
0
2
0
2
2
2
0
2
2
2
2
0
2
2
2
1
1
1
1
807
6
2
2
0
2
2
2
0
0
2
2
2
0
2
2
2
1
1
1
1
268
7
2
2
0
2
2
0
0
0
2
2
2
2
1
2
2
1
1
1
1
1041
8
2
2
0
2
2
0
0
0
0
2
2
2
1
2
2
1
1
1
1
5671, 5974
9
2
2
0
0
2
2
2
0
1
2
2
2
1
2
0
1
0
1
1
1273
10
2
2
0
2
2
2
0
0
0
2
2
2
1
2
2
0
1
1
1
827
11 (previous 8)
2
2
0
2
2
2
0
0
2
1
1
2
1
2
2
0
1
1
1
935
12
2
2
0
0
2
2
0
0
1
2
2
2
1
2
0
0
1
1
1
214, 528, 1016, 1328
13
2
2
0
2
0
2
0
0
2
1
1
2
1
2
2
0
1
1
1
425
14
2
2
0
0
2
2
0
0
1
2
2
2
1
2
0
0
1
1
0
1286
15
0
2
0
0
2
0
0
0
1
2
2
2
1
2
0
0
1
1
1
961
aPATS types are designated arbitrarily with different numbers.
bPrefixes of each PATS primer pair A/B and virulence gene primer pair F/R are indicated. 0: no amplicon; 1: amplicon without AvrII site; 2: amplicon with one XbaI or AvrII site.
cBovine E. coli O157:H7 isolates from different locations that fell within a given PATS type.
dIdentical profiles observed in previous study [18].
