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International Journal of Microbiology
Volume 2012, Article ID 575193, 3 pages
Research Article

Relationship between the Presence of the nalC Mutation and Multidrug Resistance in Pseudomonas aeruginosa

1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam 693917134, Iran
2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom, Iran

Received 10 January 2012; Revised 17 February 2012; Accepted 17 February 2012

Academic Editor: Todd R. Callaway

Copyright © 2012 Nourkhoda Sadeghifard et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objectives. The current study was conducted to determine the relationship between the presences of significant multidrug resistance in Pseudomonas aeruginosa (P. aeruginosa) having intact mexR genes (nalC) to different antibiotics. Methods. In order to identify nalC, fifty strains of P. aeruginosa were obtained. All isolates were found in urinary tract infections. They were evaluated against different antibiotics. The nalC mutant was identified by PCR. Results. The 50 clinical isolates of P. aeruginosa originated from two hospitals in Iran, in which 32 isolates were found in Milad hospital, and 18 isolates were collected in the Ilam Hospital. The results in Milad hospital of nalC revealed that all P. aeruginosa resistant to oxacillin showed the presence of nalC. In Ilam hospital only three (16.6%) isolates were resistant to oxacilin and aztreonam, and among these three isolates only one isolate revealed resistance to ceftazidime and amikacin. The resistant isolates showed the presence of both OXA-10 and nalC. Conclusion. Our results showed that the presence of nalC was observed among P. aeruginosa resistance to oxacilin. Thus, the finding suggested relationship between oxacilin resistance and presence of nalC and consequently overproduction of the MexABOprM efflux system.