Table of Contents Author Guidelines Submit a Manuscript
International Journal of Microbiology
Volume 2012, Article ID 683193, 8 pages
http://dx.doi.org/10.1155/2012/683193
Research Article

Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation

1Department of Biotechnology Engineering, Tatyasaheb Kore Institute of Engineering & Technology, Warananagar Panhala, Kolhapur, Maharashtra, 416113, India
2Department of Microbiology, K.R.P. Kanya Mahavidyalaya, Islampur, Walwa, Sangli, Maharashtra, 415414, India

Received 8 July 2011; Revised 28 September 2011; Accepted 4 October 2011

Academic Editor: Gregory M. Cook

Copyright © 2012 Rajashri D. Kamble and Anandrao R. Jadhav. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Bataillon, A. P. Nunes Cardinali, and F. Duchiron, “Production of xylanases from a newly isolated alkalophilic thermophilic Bacillus sp,” Biotechnology Letters, vol. 20, no. 11, pp. 1067–1071, 1998. View at Publisher · View at Google Scholar · View at Scopus
  2. R. Bernier, M. Desrochers, L. Jurasek, and M. G. Paice, “Isolation and characterization of a xylanase from Bacillus subtilis,” Applied and Environmental Microbiology, vol. 46, no. 2, pp. 511–514, 1983. View at Google Scholar
  3. G. Elegir, G. Szakacs, and T. W. Jeffries, “Purification, characterization, and substrate specificities of multiple xylanases from Streptomyces sp. strain B-12-2,” Applied and Environmental Microbiology, vol. 60, no. 7, pp. 2609–2615, 1994. View at Google Scholar · View at Scopus
  4. R. D.S. Khandeparkar and N. B. Bhosle, “Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation,” Enzyme and Microbial Technology, vol. 39, no. 4, pp. 732–742, 2006. View at Publisher · View at Google Scholar
  5. Q. K. Beg, M. Kapoor, L. Mahajan, and G. S. Hoondal, “Microbial xylanases and their industrial applications: a review,” Applied Microbiology and Biotechnology, vol. 56, no. 3-4, pp. 326–338, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. N. Kulkarni, A. Shendye, and M. Rao, “Molecular and biotechnological aspects of xylanases,” FEMS Microbiology Reviews, vol. 23, no. 4, pp. 411–456, 1999. View at Publisher · View at Google Scholar · View at Scopus
  7. C. Tachaapaikoon, K. L. Kyu, and K. Ratanakhanokchai, “Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column,” Process Biochemistry, vol. 41, no. 12, pp. 2441–2445, 2006. View at Publisher · View at Google Scholar · View at Scopus
  8. S. B. Chidi, B. Godana, I. Ncube, E. J. Van Rensburg, A. Cronshaw, and E. K. Abotsi, “Production, purification and characterization of celullase-free xylanase from Aspergillus terreus UL 4209,” African Journal of Biotechnology, vol. 7, no. 21, pp. 3939–3948, 2008. View at Google Scholar · View at Scopus
  9. M. E. Flores, R. Pérez, and C. Huitrón, “β-Xylosidase and xylanase characterization and production by streptomyces sp. CH-M-1035,” Letters in Applied Microbiology, vol. 24, no. 5, pp. 410–416, 1997. View at Google Scholar
  10. A. Archana and T. Satyanarayana, “Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation,” Enzyme and Microbial Technology, vol. 21, no. 1, pp. 12–17, 1997. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Gessesse and G. Mamo, “Purification and characterization of an alkaline xylanase from alkalophilic Micrococcus sp. AR-135,” Journal of Industrial Microbiology and Biotechnology, vol. 20, pp. 210–214, 1998. View at Google Scholar
  12. S. Nakamura, K. Wakabayashi, R. Nakai, R. Aono, and K. Horikoshi, “Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1,” Applied and Environmental Microbiology, vol. 59, no. 7, pp. 2311–2316, 1993. View at Google Scholar · View at Scopus
  13. S. Ninawe, M. Kapoor, and R. C. Kuhad, “Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32,” Bioresource Technology, vol. 99, no. 5, pp. 1252–1258, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. N. Saitou and M. Nei, “The neighbor-joining method: a new method for reconstructing phylogenetic trees,” Molecular Biology and Evolution, vol. 4, no. 4, pp. 406–425, 1987. View at Google Scholar · View at Scopus
  15. D. Shallom and Y. Shoham, “Microbial hemicellulases,” Current Opinion in Microbiology, vol. 6, no. 3, pp. 219–228, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. M. C. Srinivasan and M. V. Rele, “Microbial xylanases for paper industry,” Current Science, vol. 77, no. 1, pp. 137–142, 1999. View at Google Scholar
  17. L. Viikari, A. Kantelinen, K. Poutanen, and M. Ranua, “Characterization of pulps treated with hemicellulolytic enzymes prior to bleaching,” in Biotechnology in Pulp and Paper Manufacture, T. K. Kirk and H. M. Chang, Eds., pp. 145–151, Butterworth-Heinemann, Stoneham, Mass, USA, 1990. View at Google Scholar
  18. M. Ayyachamy and T. M. Vatsala, “Production and partial characterization of cellulase free xylanase by Bacillus subtilis C 01 using agriresidues and its application in biobleaching of nonwoody plant pulps,” Letters in Applied Microbiology, vol. 45, no. 5, pp. 467–472, 2007. View at Publisher · View at Google Scholar · View at Scopus
  19. L. N. Ten, W. T. Im, M. K. Kim, M. S. Kang, and S. T. Lee, “Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates,” Journal of Microbiological Methods, vol. 56, no. 3, pp. 375–382, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. P. H. A. Sneath, “Endospore forming Gram positive rods and cocci,” in Bergey’s Manual of Systematic Bacteriology, W. M. Hensyl, Ed., Williams & Wilkins, Philadelphia, Pa, USA, 9th edition, 1994. View at Google Scholar
  21. M. J. Bailey, P. Biely, and K. Poutanen, “Interlaboratory testing of methods for assay of xylanase activity,” Journal of Biotechnology, vol. 23, no. 3, pp. 257–270, 1992. View at Publisher · View at Google Scholar · View at Scopus
  22. G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959. View at Google Scholar · View at Scopus
  23. T. K. Ghose, “Measurement of cellulase activities,” Pure and Applied Chemistry, vol. 59, pp. 257–268, 1987. View at Google Scholar
  24. A. H. Lachke, “1,4-β-d-Xylan xylohydrolase of Sclerotium rolfsii,” Methods in Enzymology, vol. 160, no. C, pp. 679–684, 1988. View at Publisher · View at Google Scholar · View at Scopus
  25. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  26. V. Jagannathan, K. Singh, and M. Damodaran, “Carbohydrate metabolism in citric acid fermentation. 4. Purification and properties of aldolase from Aspergillus niger,” The Biochemical Journal, vol. 63, no. 1, pp. 94–105, 1956. View at Google Scholar · View at Scopus
  27. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  28. H. Lineweaver and D. Burk, “The determination of enzyme dissociation constants,” Journal of the American Chemical Society, vol. 56, no. 3, pp. 658–666, 1934. View at Google Scholar · View at Scopus
  29. W. E. Trevelyan, D. P. Procter, and J. S. Harrison, “Detection of sugars on paper chromatograms,” Nature, vol. 166, no. 4219, pp. 444–445, 1950. View at Publisher · View at Google Scholar · View at Scopus
  30. S. Shivaji, K. Suresh, P. Chaturvedi, S. Dube, and S. Sengupta, “Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India,” International Journal of Systematic and Evolutionary Microbiology, vol. 55, no. 3, pp. 1123–1127, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. J. X. Heck, L. H. D. B. Soares, P. F. Hertz, and M. A. Z. Ayub, “Purification and properties of a xylanase produced by Bacillus circulans BL53 on solid-state cultivation,” Biochemical Engineering Journal, vol. 32, no. 3, pp. 179–184, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. K. Malarvizhi, K. Murugesan, and P. T. Kalaichelvan, “Xylanase production by Ganoderma lucidum on liquid and solid state fermentation,” Indian Journal of Experimental Biology, vol. 41, no. 6, pp. 620–626, 2003. View at Google Scholar · View at Scopus
  33. P. V. Gawande and M. Y. Kamat, “Production of Aspergillus xylanase by lignocellulosic waste fermentation and its application,” Journal of Applied Microbiology, vol. 87, no. 4, pp. 511–519, 1999. View at Publisher · View at Google Scholar · View at Scopus
  34. D. Haltrich, B. Nidetzky, K. D. Kulbe, W. Steiner, and S. Župančič, “Production of fungal xylanases,” Bioresource Technology, vol. 58, no. 2, pp. 137–161, 1996. View at Publisher · View at Google Scholar
  35. W. Panbangred, A. Shinmyo, S. Kinoshita, and H. Okada, “Purification and properties of endoxylanase produced by Bacillus pumilus,” Agricultural and Biological Chemistry, vol. 47, no. 5, pp. 957–963, 1983. View at Google Scholar
  36. G. M. Zhang, J. Huang, G. R. Huang, L. X. Ma, and X. E. Zhang, “Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina,” Applied Microbiology and Biotechnology, vol. 74, no. 2, pp. 339–346, 2007. View at Publisher · View at Google Scholar · View at Scopus
  37. A. Blanco, T. Vidal, J. F. Colom, and F. I. J. Pastor, “Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23,” Applied and Environmental Microbiology, vol. 61, no. 12, pp. 4468–4470, 1995. View at Google Scholar
  38. M. J. Tseng, M. N. Yap, K. Ratanakhanokchai, K. L. Kyu, and S. T. Chen, “Purification and characterization of two cellulase free xylanases from an alkaliphilic Bacillus firmus,” Enzyme and Microbial Technology, vol. 30, no. 5, pp. 590–595, 2002. View at Publisher · View at Google Scholar · View at Scopus
  39. M. Ujiie, C. Roy, and M. Yaguchi, “Low-molecular-weight xylanase from Trichoderma viride,” Applied and Environmental Microbiology, vol. 57, no. 6, pp. 1860–1862, 1991. View at Google Scholar · View at Scopus
  40. T. Kanda, Y. Amano, and K. Nisizawa, “Purification and properties of two endo-1,4-β-xylanases from Irpex lacteus (Polyporus tulipiferae),” Journal of Biochemistry, vol. 98, no. 6, pp. 1545–1554, 1985. View at Google Scholar · View at Scopus
  41. C. Y. Wang, H. Chan, H. T. Lin, and Y. T. Shyu, “Production, purification and characterisation of a novel halostable xylanase from Bacillus sp. NTU-06,” Annals of Applied Biology, vol. 156, no. 2, pp. 187–197, 2010. View at Google Scholar
  42. S. M. Bansod, M. Datta-Choudhary, M. C. Shrinivasan, and M. V. Rele, “Xylanase active at high pH from an alkalitolerant Cephalosporium species,” Biotechnology Letters, vol. 15, pp. 965–970, 1993. View at Google Scholar
  43. B. K. Kubata, T. Suzuki, H. Horitsu, K. Kawal, and K. Takamizawa, “Purification and characterization of Aeromonas caviae ME-1 xylanases V, which produces exclusively xylobiose from xylan,” Applied and Environmental Microbiology, vol. 60, no. 2, pp. 531–535, 1994. View at Google Scholar
  44. S. Nakamura, Y. Ishiguro, R. Nakai, K. Wakabayashi, R. Aono, and K. Horikoshi, “Purification and characterization of a thermophilic alkaline xylanase from thermoalkaliphilic Bacillus sp. strain TAR-1,” Journal of Molecular Catalysis B, vol. 1, no. 1, pp. 7–15, 1995. View at Publisher · View at Google Scholar · View at Scopus
  45. L. U. L. Tan, P. Mayers, and J. N. Saddler, “Purification and characterization of a thermostable xylanase from a thermophilic fungus Thermoascus aurantiacus,” Canadian Journal of Microbiology, vol. 33, no. 8, pp. 689–692, 1987. View at Google Scholar
  46. T. Nanmori, T. Watanabe, R. Shinke, A. Kohno, and Y. Kawamura, “Purification and properties of thermostable xylanase and β-xylosidase produced by a newly isolated Bacillus stearothermophilus strain,” Journal of Bacteriology, vol. 172, no. 12, pp. 6669–6672, 1990. View at Google Scholar · View at Scopus
  47. H. Honda, T. Kudo, Y. Ikura, and K. Horikoshi, “Two types of xylanases of alkalophilic Bacillus sp. No. C-125,” Canadian Journal of Microbiology, vol. 31, no. 6, pp. 538–542, 1985. View at Google Scholar · View at Scopus
  48. A. Ohkoshi, T. Kudo, T. Mase, and K. Horikoshi, “Purification of three types of xylanases from an alkaliphilic Aeromonas sp,” Agricultural and Biological Chemistry, vol. 49, no. 10, pp. 3037–3038, 1985. View at Google Scholar
  49. M. K. Kang, P. J. Maeng, and Y. H. Rhee, “Purification and characterization of two xylanases from alkaliphilic Cephalosporium sp. strain RYM-202,” Applied and Environmental Microbiology, vol. 62, no. 9, pp. 3480–3482, 1996. View at Google Scholar
  50. N. Hakulinen, O. Turunen, J. Jänis, M. Leisola, and J. Rouvinen, “Three-dimensional structures of thermophilic β-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa: comparison of twelve xylanases in relation to their thermal stability,” European Journal of Biochemistry, vol. 270, no. 7, pp. 1399–1412, 2003. View at Publisher · View at Google Scholar · View at Scopus