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International Journal of Microbiology
Volume 2012, Article ID 865175, 5 pages
Research Article

Use of Self-Organizing Map to Analyze Images of Fungi Colonies Grown from Triticum aestivum Seeds Disinfected by Ozone Treatment

1Centre pour l'Agronomie et l'Agro-Industrie de la Province de Hainaut (CARAH), 11 rue P. Pastur, 7800 Ath, Wallonie, Belgium
2Haute École Provinciale de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonie, Belgium

Received 5 March 2012; Revised 20 September 2012; Accepted 20 September 2012

Academic Editor: Joseph Falkinham

Copyright © 2012 Thierry Marique et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We submitted to ozone treatment Triticum aestivum (common wheat) seeds severely contaminated by fungi. Fungi colonies developed when seeds were placed over malt agar medium in Petri dishes; Fusarium sp. and Alternaria sp. were identified. However, conventional colonies counting did not allow a clear assessment of the effect of ozone disinfection. We thus used self-organizing maps (SOMs) to perform an image analysis of colonies surface area that clearly showed a significant disinfection effect on Fusarium sp.