Table of Contents Author Guidelines Submit a Manuscript
International Journal of Microbiology
Volume 2013 (2013), Article ID 276921, 8 pages
http://dx.doi.org/10.1155/2013/276921
Research Article

Taxonomy and Polyphasic Characterization of Alkaline Amylase Producing Marine Actinomycete Streptomyces rochei BTSS 1001

1Department of Biotechnology, Dr. L. Bullayya College, New Resapuvanipalem, Visakhapatnam, Andhra Pradesh 530013, India
2Department of Biotechnology, Andhra University, Visakhapatnam 530003, India
3School of Life Sciences, GITAM University, Visakhapatnam 530045, India

Received 13 July 2013; Accepted 7 October 2013

Academic Editor: David C. Straus

Copyright © 2013 Aparna Acharyabhatta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. T. J. Mincer, P. R. Jensen, C. A. Kauffman, and W. Fenical, “Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments,” Applied and Environmental Microbiology, vol. 68, no. 10, pp. 5005–5011, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. J. E. M. Stach, L. A. Maldonado, D. G. Masson, A. C. Ward, M. Goodfellow, and A. T. Bull, “Statistical approaches for estimating actinobacterial diversity in marine sediments,” Applied and Environmental Microbiology, vol. 69, no. 10, pp. 6189–6200, 2003. View at Publisher · View at Google Scholar · View at Scopus
  3. P. R. Jensen and W. Fenical, “New natural-product diversity from marine actinomycetes,” in Natural Products: Drug Discovery and Therapeutics Medicines, L. Zhang and A. Demain, Eds., vol. 5, pp. 315–328, Humana Press, 2005. View at Google Scholar
  4. L. Zhang, R. An, J. Wang et al., “Exploring novel bioactive compounds from marine microbes,” Current Opinion in Microbiology, vol. 8, no. 3, pp. 276–281, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. K. S. Lam, “Discovery of novel metabolites from marine actinomycetes,” Current Opinion in Microbiology, vol. 9, no. 3, pp. 245–251, 2006. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Dharmaraj, “Marine Streptomyces as a novel source of bioactive substances,” World Journal of Microbiology and Biotechnology, vol. 26, no. 12, pp. 2123–2139, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. T. W. Hodges, M. Slattery, and J. B. Olson, “Unique actinomycetes from marine caves and coral reef sediments provide novel PKS and NRPS biosynthetic gene clusters,” Marine Biotechnology, vol. 14, no. 3, pp. 270–280, 2012. View at Publisher · View at Google Scholar · View at Scopus
  8. A. T. Bull, A. C. Ward, and M. Goodfellow, “Search and discovery strategies for biotechnology: the paradigm shift,” Microbiology and Molecular Biology Reviews, vol. 64, no. 3, pp. 573–606, 2000. View at Google Scholar · View at Scopus
  9. N. A. Magarvey, J. M. Keller, V. Bernan, M. Dworkin, and D. H. Sherman, “Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites,” Applied and Environmental Microbiology, vol. 70, no. 12, pp. 7520–7529, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. A. S. Anderson and E. M. H. Wellington, “The taxonomy of Streptomyces and related genera,” International Journal of Systematic and Evolutionary Microbiology, vol. 51, no. 3, pp. 797–814, 2001. View at Google Scholar · View at Scopus
  11. A. Pandey, P. Nigam, C. R. Soccol, V. T. Soccol, D. Singh, and R. Mohan, “Advances in microbial amylases,” Biotechnology and Applied Biochemistry, vol. 31, no. 2, pp. 135–152, 2000. View at Google Scholar · View at Scopus
  12. M. J. van der Maarel, B. van der Veen, J. Uitdehaag, H. Leemhuis, and L. Dijkhuizen, “Properties and applications of starch-converting enzymes of the α-amylase family,” Journal of Biotechnology, vol. 94, no. 2, pp. 137–155, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. H. W. G. Kuilderd, “Applied technology-simultaneous desizing and scouring with enzymes-simultaneous fabric desizing and scouring, using alkaline alpha-amylase and an alkaline scouring enzyme, reduces water,” American Association of Textile Chemists and Colorists, vol. 8, pp. 33–36, 2008. View at Google Scholar
  14. M. Antoinette Mc Tigue, C. T. Kelly, E. M. Doyle, and W. M. Fogarty, “The alkaline amylase of the alkalophilic Bacillus sp. IMD 370,” Enzyme and Microbial Technology, vol. 17, no. 6, pp. 570–573, 1995. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Yang, L. Liu, J. Li, G. Du, and J. Chen, “Heterologous expression, biochemical characterization, and overproduction of alkaline alpha-amylase from Bacillus alcalophilus in Bacillus subtilis,” Microbial Cell Factories, vol. 10, article 77, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Burhan, U. Nisa, C. Gökhan, C. Ömer, A. Ashabil, and G. Osman, “Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6,” Process Biochemistry, vol. 38, no. 10, pp. 1397–1403, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. S. O. Hashim, O. D. Delgado, M. A. Martínez, R.-H. Kaul, F. J. Mulaa, and B. Mattiasson, “Alkaline active maltohexaose-forming α-amylase from Bacillus halodurans LBK 34,” Enzyme and Microbial Technology, vol. 36, no. 1, pp. 139–146, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. N. Hmidet, A. Bayoudh, J. G. Berrin, S. Kanoun, N. Juge, and M. Nasri, “Purification and biochemical characterization of a novel α-amylase from Bacillus licheniformis NH1. Cloning, nucleotide sequence and expression of amyN gene in Escherichia coli,” Process Biochemistry, vol. 43, no. 5, pp. 499–510, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. B. Arikan, “Highly thermostable, thermophilic, alkaline, SDS and chelator resistant amylase from a thermophilic Bacillus sp. isolate A3-15,” Bioresource Technology, vol. 99, no. 8, pp. 3071–3076, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. B. H. Joshi, “A novel thermostable alkaline α-amylase from bacillus circulans PN5: biochemical characterization and production,” Asian Journal of Biotechnology, vol. 3, pp. 58–67, 2007. View at Google Scholar
  21. D. G. Syed, D. Agasar, and A. Pandey, “Production and partial purification of α-amylase from a novel isolate Streptomyces gulbargensis,” Journal of Industrial Microbiology and Biotechnology, vol. 36, no. 2, pp. 189–194, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. R. K. Saxena, K. Dutt, L. Agarwal, and P. Nayyar, “A highly thermostable and alkaline amylase from a Bacillus sp. PN5,” Bioresource Technology, vol. 98, no. 2, pp. 260–265, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. R. Nakai, T. Sato, and K. Okamoto, “Manufacture of alkaline amylase with Streptomyces,” Japanese Kokai Tokkyo Koho, vol. 86, no. 209, 588, 1986. View at Google Scholar
  24. S. Chakraborty, R. G. A. Khopade, K. Mahadik, and C. Kokare, “Study on calcium ion independent α -amylase from haloalkaliphilic marine Streptomyces strain A3,” Indian Journal of Biotechnology, vol. 11, JP2000060546A2, pp. 427–437, 2012. View at Google Scholar
  25. S. Chakraborty, A. Khopade, C. Kokare, K. Mahadik, and B. Chopade, “Isolation and characterization of novel α-amylase from marine Streptomyces sp. D1,” Journal of Molecular Catalysis B, vol. 58, no. 1–4, pp. 17–23, 2009. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Chakraborty, R. G. A. Khopade, K. Mahadik, and C. Kokare, “Study on calcium ion independent α -amylase from haloalkaliphilic marine Streptomyces strain A3,” Indian Journal of Biotechnology, vol. 11, pp. 427–437, 2012. View at Google Scholar
  27. S. Ramesh and N. Mathivanan, “Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes,” World Journal of Microbiology and Biotechnology, vol. 25, no. 12, pp. 2103–2111, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. J. Berger, L. M. Jampolsky, and M. W. Goldberg, “A guide to the classification of the actinomycetes and their antibiotics,” in A Guide to the Classification of the Actinomycetes and their Antibiotics, S. A. W. Lechevalier and H. A. Baltimore, Eds., pp. 1–246, The Williams & Wilkins, 1953. View at Google Scholar
  29. G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959. View at Google Scholar · View at Scopus
  30. O. H. Lowry, N. J. Rosenbrough, A. L. Fan, and R. J. Randall, “Protein estimation with the Folinphenol reagent,” Indian Journal of Biotechnology, vol. 193, pp. 265–275, 1951. View at Google Scholar
  31. E. B. Shirling and D. Gottlieb, “Methods for characterization of Streptomyces species,” International Journal of Systematic and Evolutionary Microbiology, vol. 16, pp. 313–340, 1966. View at Google Scholar
  32. M. Goodfellow, “Numerical taxonomy of some nocardioform bacteria,” Journal of General Microbiology, vol. 69, no. 1, pp. 33–80, 1971. View at Google Scholar · View at Scopus
  33. R. E. Gordon, D. A. Barnett, J. E. Handerhan, and C. Hor Nay Pang, “Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strain,” International Journal of Systematic Bacteriology, vol. 24, no. 1, pp. 54–63, 1974. View at Google Scholar · View at Scopus
  34. S. T. Williams, S. Lanning, and E. M. H. Wellington, “Ecology of actinomycetes,” in The Biology of the Actinomycetes, M. Goodfellow, M. Mordarski, and S. T. Williams, Eds., pp. 481–528, Academic Press, London, U.K, 1984. View at Google Scholar
  35. T. Hasegawa, M. Takizawa, and S. Tanida, “A rapid analysis for chemical grouping of aerobic actinomycetes,” Journal of General and Applied Microbiology, vol. 29, no. 4, pp. 319–322, 1983. View at Google Scholar · View at Scopus
  36. J. L. Staneck and G. D. Roberts, “Simplified approach to identification of aerobic actinomycetes by thin layer chromatography,” Journal of Applied Microbiology, vol. 28, no. 2, pp. 226–231, 1974. View at Google Scholar · View at Scopus
  37. D. E. Minnikin, I. G. Hutchinson, A. B. Caldicott, and M. Goodfellow, “Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria,” Journal of Chromatography A, vol. 188, no. 1, pp. 221–233, 1980. View at Google Scholar · View at Scopus
  38. M. D. Collins, “Isoprenoid quinone analyses in bacterial classification and identification,” in Bacterial Systematics, M. Goodfellow and D. E. Minnikin, Eds., pp. 267–287, Academic Press, London, UK, 1985. View at Google Scholar
  39. M. Sasser, Technical Note 101: Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI, North Newark, Del, USA, 1991.
  40. R. M. Kroppenstedt, “Fatty acid and menaquinone analysis of actinomycetes and related organisms,” in Chemical Methods in Bacterial Systematics, M. Goodfellow and D. E. Minnikin, Eds., pp. 173–199, Elsevier Science & Technology Books, London, UK, 1985. View at Google Scholar
  41. W.-J. Li, P. Xu, P. Schumann et al., “Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia,” International Journal of Systematic and Evolutionary Microbiology, vol. 57, no. 7, pp. 1424–1428, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. A. Morgulis, G. Coulouris, Y. Raytselis, T. L. Madden, R. Agarwala, and A. A. Schäffer, “Database indexing for production MegaBLAST searches,” Bioinformatics, vol. 24, no. 16, pp. 1757–1764, 2008. View at Publisher · View at Google Scholar · View at Scopus
  43. J. D. Thompson, T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins, “The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools,” Nucleic Acids Research, vol. 25, no. 24, pp. 4876–4882, 1997. View at Publisher · View at Google Scholar · View at Scopus
  44. J. Felsenstein, “Evolutionary trees from DNA sequences: a maximum likelihood approach,” Journal of Molecular Evolution, vol. 17, no. 6, pp. 368–376, 1981. View at Google Scholar · View at Scopus
  45. J. Felsenstein, “PHYLIP (Phylogeny Inference Package) version 3.6.,” Tech. Rep., Department of Genome Sciences, University of Washington, Seattle, Wash, USA, 2005. View at Google Scholar
  46. R. Page, “TreeView: an application to display phylogenetic trees on personal computers,” Computer Applications in the Biosciences, vol. 12, no. 4, pp. 357–358, 1996. View at Google Scholar · View at Scopus
  47. J. Felsenstein, “Confidence limits on phylogenies: an approach using the bootstrap,” Evolution, vol. 39, pp. 783–791, 1985. View at Google Scholar
  48. V. S. Bernan, M. Greenstein, and W. M. Maiese, “Marine microorganisms as a source of new natural products,” Advances in Applied Microbiology, vol. 43, pp. 57–90, 1997. View at Google Scholar · View at Scopus
  49. S. Thippeswamy, K. Girigowda, and V. H. Mulimani, “Isolation and identification of α-amylase producing Bacillus sp. from dhal industry waste,” Indian Journal of Biochemistry and Biophysics, vol. 43, no. 5, pp. 295–298, 2006. View at Google Scholar · View at Scopus