|
| Primer name | Nucleotide sequence |
|
| Mutans DHPSph |
5′-GAT CGA TCG CAT GCA CAT CAT AAC TAG GGA GCA AGC-3′ |
| mutansDHPSBam | 5′-GAT GGA TCG GAT CCA AAA TAATCT TAT CCA TAA CAC CCT CA-3′ |
| dhpssfwph | 5′-AAC CTA CTG CAT GCA TAA GAA TCA G-3′ |
| dhpssreveco | 5′-ATT GTA GGA ATT CTT CTA GAA AGA TCC-3′ |
| downeifolpfw | 5′-GCA TGC CAA AGA CAG GAA TTG CTG AC-3′ |
| Downeifolprevps | 5′-CTG CAG CCA CAA AAA TTT GCC CCA GAC-3′ |
|
| | Primers for changing specific amino acids in isolate 797 |
|
| DHPS46AVfw | 5′-TGA AGC CAT GTT AGT AGC AGG AGC GGC TA-3′ |
| DHPS46AVrev | 5′-TAG CCG CTC CTG CTA CTA ACA TGG CTT CA-3′ |
| DHPS80aEKfw | 5′-TCG TTC CAA TTG TTA AAG CTA TTA GCG AA-3′ |
| DHPS80aEKrev | 5′-TTC GCT AAT AGC TTT AAC AAT TGG AAC GA-3′ |
| DHPS122QHfw | 5′-CTT TAT GAT GGG CAC ATG TTT CAA TTA GC-3′ |
| DHPS122QHrev | 5′-GCT AAT TGA AAC ATG TGC CCA TCA TAA AG-3′ |
| DHPS146SGfw | 5′-GTG AAG AAG TTT ATG GCA ATG TAA CAG AA-3′ |
| DHPS146SGrev | 5′-TTC TGT TAC ATT GCC ATA AAC TTC TTC AC-3′ |
|
| | Primers for changing specific amino acids in isolate 8 |
|
| V37Afw | 5′-AAC CAA TCG ATC AGG CTC TAA AAC AGG TTG A-3′ |
| V37Arev | 5′-TCA ACC TGT TTT AGA GCC TGA TCG ATT GTT T-3′ |
| D172Nfw | 5′-GGA GTT AAA AAA GAA AAT ATT TGG CTT GAT C-3 |
| D172Nrev | 5′-GAT CAA GCC AAA TAT TTT CTT TTT TAA CTC C-3′ |
| Q193Rfw | 5′-ACA TGG AAC TTC TAC GAG GCT TAG CGG AGG T-3 |
| Q193Rrev | 5′-ACC TCC GCT AAG CCT CGT AGA AGT TCC ATG T-3′ |
|
