International Journal of Microbiology / 2013 / Article / Tab 2

Research Article

Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans

Table 2

Primers used for cloning and site-directed mutagenesis.

Primer nameNucleotide sequence

Mutans DHPSph 5′-GAT CGA TCG CAT GCA CAT CAT AAC TAG GGA GCA AGC-3′
mutansDHPSBam5′-GAT GGA TCG GAT CCA AAA TAATCT TAT CCA TAA CAC CCT CA-3′
dhpssfwph5′-AAC CTA CTG CAT GCA TAA GAA TCA G-3′
dhpssreveco5′-ATT GTA GGA ATT CTT CTA GAA AGA TCC-3′
downeifolpfw5′-GCA TGC CAA AGA CAG GAA TTG CTG AC-3′
Downeifolprevps5′-CTG CAG CCA CAA AAA TTT GCC CCA GAC-3′

Primers for changing specific amino acids in isolate 797

DHPS46AVfw5′-TGA AGC CAT GTT AGT AGC AGG AGC GGC TA-3′
DHPS46AVrev5′-TAG CCG CTC CTG CTA CTA ACA TGG CTT CA-3′
DHPS80aEKfw5′-TCG TTC CAA TTG TTA AAG CTA TTA GCG AA-3′
DHPS80aEKrev5′-TTC GCT AAT AGC TTT AAC AAT TGG AAC GA-3′
DHPS122QHfw5′-CTT TAT GAT GGG CAC ATG TTT CAA TTA GC-3′
DHPS122QHrev5′-GCT AAT TGA AAC ATG TGC CCA TCA TAA AG-3′
DHPS146SGfw5′-GTG AAG AAG TTT ATG GCA ATG TAA CAG AA-3′
DHPS146SGrev5′-TTC TGT TAC ATT GCC ATA AAC TTC TTC AC-3′

Primers for changing specific amino acids in isolate 8

V37Afw5′-AAC CAA TCG ATC AGG CTC TAA AAC AGG TTG A-3′
V37Arev5′-TCA ACC TGT TTT AGA GCC TGA TCG ATT GTT T-3′
D172Nfw5′-GGA GTT AAA AAA GAA AAT ATT TGG CTT GAT C-3
D172Nrev5′-GAT CAA GCC AAA TAT TTT CTT TTT TAA CTC C-3′
Q193Rfw5′-ACA TGG AAC TTC TAC GAG GCT TAG CGG AGG T-3
Q193Rrev5′-ACC TCC GCT AAG CCT CGT AGA AGT TCC ATG T-3′