Serendipitous Isolation of Non-Vibrio Bacterial Strains Carrying the Cholera Toxin Gene from Environmental Waters in Indonesia

Figure 1

Comparison of PCR and nested PCR targeting ctxA with template DNA prepared from a commercial whole genome DNA extraction kit ((A1), 1st-PCR; (A2), nested PCR) and a commercial plasmid DNA extraction kit ((B1), 1st-PCR; (B2), nested PCR). Lane 1, GCDV 10-1; lane 2, GCDV 10-2; lane 3, GCDV 31-1; lane 4, GCDV 34-3; lane 5, GCDV 47-1; lane 6, GCDV 28-2; lane 7, GCDV 37-1; lane 8, GCDV 50-6; lane P, Vibrio cholerae N16961 (positive control); lane M, 100 bp ladder size marker; lane N, PCR negative control.