Serendipitous Isolation of Non-Vibrio Bacterial Strains Carrying the Cholera Toxin Gene from Environmental Waters in Indonesia

Table 1

Primer sequences used for the sets of PCR amplification. All primers were designed on the basis of the sequence of V. cholerae O1 El Tor N16961 (Genbank accession no. AE003852).

Genes	Usage	Sequence
		Forward (5′-3′)	Reverse (5′-3′)
ctxB	1st-PCR; nested PCR	AGCATATGCACATGGAACACC TGTGTGCAGAATACCACAACA	AATTGCGGCAATCGCATGA GCAATCCTCAGGGTATCCTT
ctxA	1st-PCR; nested PCR	CGGGCAGATTCTAGACCTCCTG GCAGTCAGGTGGTCTTATGCC	CGATGATCTTGGAGCATTCCCAC GCATGATGAATCCACGGCTC
zot	1st-PCR; nested PCR	TCGCTTAACGATGGCGCGTTTT GATGAATGTGGTCGCATCTGG	AACCCCGTTTCACTTCTACCCA ATCCGGTAACGGTAGCACCTT
ace	1st-PCR; nested PCR	TAAGGATGTGCTTATGATGGACACCC	CGTGATGAATAAAGATACTCATAGG
		TGGCTTGTGATCAAGCTCG	CATCAAAGCCTGAAGCACG
orfU	1st-PCR; nested PCR	GGTGTTATTTGATGGCTGCATG ACTTGTCCGGAGCATCTGCA	AAACATCAATGCGGATTTCCTC TGACTGCGGTGACACTCTCAA
cep	1st-PCR; nested PCR	GCTACATGTTTAGCTCACTG	TTTAGCCTTACGAATTAAGCC
		GCTTTCACTCGGGGTTTTCT	TCAGAACAATTGCCCCCA
rstB	1st-PCR; nested PCR	CTCTCATTCTGAAGGGGTGAGT GTTTGACGTTTGGCTTGCAG	AGGCTTATCCAATGGCTTGC AGGTTGCGTGATGGGTCTT
rstA	1st-PCR; nested PCR	GCAGATTTTCACTCTTGACGAA GAAGTGGAACGTTGCCATGA	GGTTGAGTGAATCGTCGTGAAT CACCTTGACAGGCAAGGAAT
rstR	1st-PCR; nested PCR	CTAGCCAACCAAAGAAAGGCA GGGGAAGGTTTGCCTACAAT	GCACCATGATTTAAGATGCTCTTG CCCATCTTCCGCATAGTTCA
tcpA	PCR	AGAAAACCGGTCAAGAGGG	CTGTGAATGGAGCAGTTCCTG
tolQ	PCR	TCGTGGGCGGCAATTATCA	AGCCATCACTTGACGGTGGAG
tolR	PCR	TGGCTGGCTATCAAACCAA	TTTAAGGTCCGTGAGTAGCCC
tolA	PCR	CGGTGCTTTGGTCGCGATAT	CGTCAGGTTGATCTTTCGGCA