Bacteriophage-Derived Peptidase Eliminates and Prevents Staphylococcal Biofilms
Figure 5
Skin decolonisation. Reduction of S. aureus on porcine skin using . (i) Two sections of porcine skin were seeded with 2.5 × 105 CFU cm−2 of S. aureus DPC 5246. Plate counts were performed to enumerate the S. aureus present on porcine skin 30 min after being sprayed with either sterile water or solution. (ii) Two sections of skin were seeded with 2.4 × 106 CFU cm−2 of S. aureus Xen29. One area of skin was treated with sterile water (a) and the other area treated with solution (b). Skin was imaged (exposure: 15 seconds, binning: medium, colour-scale 36–67) 30 min after treatment with /H2O using the in vivo Imaging System (IVIS) (Xenogen, Ca). This system enabled direct monitoring of the effect of treatment in real time. The colour bar represents bacterial bioluminescence signal intensity, with red to blue indicating high and low bioluminescence, respectively.
