Soil Acidobacterial 16S rRNA Gene Sequences Reveal Subgroup Level Differences between Savanna-Like Cerrado and Atlantic Forest Brazilian Biomes

Figure 6

Acidobacterial phylogenetic reconstruction with representative OTUs using 85% similarity cutoff obtained with MEGA 5.05, using the neighbor-joining method, the Jukes-Cantor model of substitution, and a bootstrap of 1,000. Bootstrap values are shown for those branches with >50% support. The scale bar indicates the similarity distance between branches. Blank circles and squares represent exclusive OTUs for “Serra dos Órgãos” and “Serra do Mar,” respectively. Inverted triangles represent OTUs shared by all three areas (Cerrado, “Serra dos Órgãos,” and “Serra do Mar”: SA, SO, SM). Each line in the phylogenetic tree shows first the NCBI accession number for the representative sequence, second the region of the Venn Diagram from which the representative OTU is derived unique from “Serra dos Órgãos” (SO), exclusive from “Serra do Mar” (SM), or shared from two or three areas (i.e. Savanna-Serra dos Órgãos (SA-SO)), third in parenthesis the soil subarea where the representative was sampled (Cerrado (SA): “Mata de galeria” (MG), “campo sujo” (CS), “Cerrado sensu stricto” (SS), “Cerrado denso” (CD); “Serra dos Órgãos” (SO): sites 1 to 6 (S1, S2, S3, S4, S5, S6); “Serra do Mar” (SM): MA01, MA02, MA03, MA04, MA05, MA06, MA07, MA08, MA09, MA10), fourth in parenthesis the number of sequences represented by the OTU, and fifth the acidobacterial clusters of subgroups marked according to the name of the group.