International Journal of Microbiology / 2014 / Article / Tab 2

Research Article

Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction

Table 2

Hybridization signals of average of all probe sets and seven selected probe sets and fold changes of seven selenite-responsive candidate genes from microarray analysis.

Probe set IDaGene name Signals/encoding enzymesMicroarray hybridization signalsbFold changesc
0 0.01 mM5 mM0.01 mM5 mM

       Average of all probe sets554.2 ± 2.3557.0 ± 1.9560.2 ± 1.8

1762328glmM Phosphoglucosamine mutase61.0 ± 15.464.3 ± 6.832.0 ± 4.71.1−1.9
1764010rfaP Lipopolysaccharide core biosynthesis protein rfaP33.7 ± 1.751.8 ± 9.549.4 ± 1.61.51.5
1764732yegB Multidrug efflux system protein MdtE27.0 ± 10.555.4 ± 3.748.9 ± 162.21.8
1767270ydbA Hypothetical protein144.8 ± 17.690.4 ± 3.8135.3 ± 18.2−1.61.1
1768816ynbC Hypothetical protein55.5 ± 4.632.0 ± 942.6 ± 6.1−1.81.3
1768993phnL PhnI protein50.6 ± 10.170.1 ± 14.290.7 ± 151.41.8
1768842nfi Endonuclease V43.9 ± 6.372.1 ± 1543.3 ± 11.31.61.0

Selected probe sets corresponding to selenite-responsive candidate genes have absolute fold changes ≥1.5 and -test values ≤ 0.05 either in 0.01 or 5 mM Na2SeO3 treatment. bHybridization signals of average of all 10,208 probe sets and seven probe sets. Mean values ± SD . Average of background signals are 39.7 ± 2.3, 45.8 ± 1, and 47.4 ± 0.4 for 0, 0.01 and 5 mM, respectively. cFold changes of selenite-responsive candidate genes from microarray analysis comparing to untreated control. “−” means reduced expression.

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