Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction

Table 2

Hybridization signals of average of all probe sets and seven selected probe sets and fold changes of seven selenite-responsive candidate genes from microarray analysis.

Probe set ID^a	Gene name	Signals/encoding enzymes	Microarray hybridization signals^b			Fold changes^c
			0	0.01 mM	5 mM	0.01 mM	5 mM
		Average of all probe sets	554.2 ± 2.3	557.0 ± 1.9	560.2 ± 1.8
1762328	glmM	Phosphoglucosamine mutase	61.0 ± 15.4	64.3 ± 6.8	32.0 ± 4.7	1.1	−1.9
1764010	rfaP	Lipopolysaccharide core biosynthesis protein rfaP	33.7 ± 1.7	51.8 ± 9.5	49.4 ± 1.6	1.5	1.5
1764732	yegB	Multidrug efflux system protein MdtE	27.0 ± 10.5	55.4 ± 3.7	48.9 ± 16	2.2	1.8
1767270	ydbA	Hypothetical protein	144.8 ± 17.6	90.4 ± 3.8	135.3 ± 18.2	−1.6	1.1
1768816	ynbC	Hypothetical protein	55.5 ± 4.6	32.0 ± 9	42.6 ± 6.1	−1.8	1.3
1768993	phnL	PhnI protein	50.6 ± 10.1	70.1 ± 14.2	90.7 ± 15	1.4	1.8
1768842	nfi	Endonuclease V	43.9 ± 6.3	72.1 ± 15	43.3 ± 11.3	1.6	1.0

aSelected probe sets corresponding to selenite-responsive candidate genes have absolute fold changes ≥1.5 and 𝑡-test 𝑃 values ≤ 0.05 either in 0.01 or 5 mM Na₂SeO₃ treatment. ^bHybridization signals of average of all 10,208 probe sets and seven probe sets. Mean values ± SD (𝑛=3). Average of background signals are 39.7 ± 2.3, 45.8 ± 1, and 47.4 ± 0.4 for 0, 0.01 and 5 mM, respectively. ^cFold changes of selenite-responsive candidate genes from microarray analysis comparing to untreated control. “−” means reduced expression.