Antibiofilm Activity, Compound Characterization, and Acute Toxicity of Extract from a Novel Bacterial Species of Paenibacillus

Table 2

Antibiofilm activity of potential compounds of 139SI against Gram-positive clinical isolates.

Experimental treatment	Staphylococcus aureus	Streptococcus agalactiae	Group G Streptococci	Streptococcus pyogenes	Streptococcus pneumoniae	Biofilm-forming strain S. aureus; (ATCC 25923)	Nonbiofilm-forming strain E. coli; (ATCC 25922)
	OD ± SD	OD ± SD	OD ± SD	OD ± SD	OD ± SD	OD ± SD	OD ± SD
With compound FR4	0.254±0.004	0.205±0.005	0.199±0.010	0.191±0.003	0.230±0.004	0.246±0.004	0.186±0.004
Without compound	0.484±0.008	0.215±0.002	0.253±0.002	0.304±0.003	0.371±0.002	0.395±0.003	0.216±0.073
With compound FR5*	0.224±0.004	0.166±0.005	0.158±0.002	0.153±0.005	0.224±0.004	0.166±0.005	0.158±0.002
Without compound	0.368±0.028	0.304±0.003	0.377±0.122	0.363±0.079	0.445±0.042	0.030±0.038	0.224±0.005
With compound FR13	0.208±0.004	0.205±0.004	0.195±0.004	0.216±0.004	0.235±0.004	0.254±0.004	0.165±0.004
Without compound	0.404±0.003	0.276±0.056	0.262±0.003	0.257±0.024	0.277±0.002	0.378±0.003	0.254±0.003
With 2(5H)-furanone (+ve control)	0.119±0.002	0.123±0.003	0.116±0.004	0.117±0.001	0.121±0.001	0.107±0.004	0.116±0.002
Without compound	0.257±0.003	0.216±0.005	0.206±0.055	0.202±0.099	0.214±0.030	0.216±0.005	0.211±0.002
With BHI broth (−ve control)	0.057±0.038	0.050±0.006	0.021±0.002	0.069±0.020	0.095±0.012	0.084±0.006	0.084±0.006
Without compound	0.254±0.003	0.243±0.045	0.211±0.002	0.243±0.045	0.270±0.042	0.243±0.002	0.208±0.074

OD >0.24 is positive biofilm former isolate.
OD >0.12–<0.24 is weak biofilm former isolate.
OD <0.12 is negative biofilm former isolate.
*The most active compound.