Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

Table 2

Combined spa gene typing and HaeII RFLP patterns of the 75 isolated MRSA strains.

spa band types (4)	spa gene subtypes (11)	RFLP pattern By HaeII (12)	Size of PCR product (approximate bp)	Size of HaeII fragments (approximate bp)	Total isolates number (%)	Isolate serial number
S1 (1 band); 63/75 (84%)	S1a	A1 (uncut)	192	192	1 (1.3)	43
	S1b	B1	1104	2 (240), 600	3 (4)	44, 69, 70
	S1c	B2	1200	2 (240), 696	21 (28)	1–3, 15, 17, 45, 47, 48, 50, 57–59, 63, 66, 67, 71–75
		B3	1200	2 (192), 792	1 (1.3)	55
	S1d	B4	1392	2 (240), 912	37 (49.3)	4–13, 18–35, 37, 38, 40–42, 51–54
S2 (2 bands); 7/75 (9.3%)	S2a	A2 (uncut)	144, 600	144, 600	1 (1.3)	64
	S2b	C1	408, 1296	2 (192), 312, 1008	1 (1.3)	14
	S2c	B5	240, 1200	2 (240), 912	3 (4)	60, 61, 65
	S2d	C2	240, 1392	2 (240), 504, 792	1 (1.3)	62
	S2e	C3	360, 1392	100, 240, 504, 912	1 (1.3)	68
S3 (3 bands); 1/75 (1.3%)	S3	D	360, 1104, 1392	150, 2 (240), 792, 456, 912	1 (1.3)	36
S4 (no band); 4/75 (5.3%)	S4; No band	E	—	—	4 (5.3)	39, 46, 49, 56