High Doses of Halotolerant Gut-Indigenous Lactobacillus plantarum Reduce Cultivable Lactobacilli in Newborn Calves without Increasing Its Species Abundance

Figure 2

Selective microbial enumeration and colonization of L. plantarum Lplant-B80 following oral administration of low- and high doses in neonatal calves. (a) The use of optical density for quantification of bacterial growth in broth allowed the identification of optimal acid and salt concentrations to favor the optimal growth of as few as 10² CFU of Lplant-B80 in an acid/salt-MRS broth, under aerobic conditions, preventing the growth of most previously tested LAB in our laboratory; see [9]. (b) Large distinct colonies for Lplant-B80 on MRS-salt agar, from a fraction of freeze-dried pellet stored for 48 months at room temperature and rehydrated with PBS. (c) Binary analysis of fecal colonization with L. plantarum Lplant-B80 before, during, and after administration. 1, recovered; 0, not recovered. Note similar overall recovery rate (histograms) across groups and high recovery during treatment period.

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