Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome

<div>Effects of water-miscible organic solvent on <svg height="12.7484pt" id="M94" style="vertical-align:-3.981999pt" version="1.1" viewbox="-0.0498162 -8.7664 50.1255 12.7484" width="50.1255pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M655 0V28C585 35 563 50 517 120C469 193 414 280 360 366C427 469 460 516 481 545C526 604 545 614 619 622V650H391V622C457 614 462 604 438 560C411 511 381 463 339 398C295 466 258 523 225 580C210 606 211 615 272 622V650H26V622C98 614 111 603 154 538C189 482 235 408 288 324C200 188 149 116 124 85C95 47 64 34 7 28V0H236V28C169 36 164 46 188 89C220 148 261 217 310 291C372 193 417 118 442 77C461 44 454 35 392 28V0H655Z" id="g121-86"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="654" vert-adv-y="654" xpluscharwidth="8.502"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,7.81,0)"><path d="M478 437H297V411C355 404 361 392 349 355C329 292 285 174 257 106L167 356C153 394 158 404 205 411L204 437H2V411C57 404 66 391 88 336C117 265 162 144 202 30C208 13 209 0 204 -15C199 -28 191 -49 173 -84C149 -128 130 -153 98 -172C81 -184 73 -193 73 -209C73 -233 101 -257 129 -257C143 -257 156 -252 161 -232C175 -179 197 -122 264 38C333 203 364 286 390 339C417 395 425 403 478 411V437Z" id="g121-119"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="459" vert-adv-y="459" xpluscharwidth="13.777"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,14.064,0)"><path d="M524 0V26C466 32 460 36 460 104V297C460 393 411 449 331 449C302 449 276 437 248 419C223 402 201 387 181 372V451C137 432 90 420 42 411V388C96 378 102 374 102 310V104C102 38 97 33 29 26V0H246V26C187 32 181 36 181 104V339C211 365 250 390 290 390C357 390 381 345 381 276V109C381 40 374 32 315 26V0H524Z" id="g121-108"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="547" vert-adv-y="547" xpluscharwidth="21.175"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,21.176,0)"><path d="M673 0V28C608 34 594 43 563 129C499 303 432 494 370 665L339 656L132 132C98 43 84 37 20 28V0H238V28C162 35 154 45 175 108C188 150 203 192 219 237H432C454 176 474 122 487 83S491 36 428 28V0H673ZM418 280H234C265 362 296 450 328 535H330L418 280Z" id="g121-63"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="691" vert-adv-y="691" xpluscharwidth="30.159"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,30.273,3.818)"><path d="M871 0V33C783 41 780 45 777 152L768 506C765 610 771 609 860 617V650H683L453 157L228 650H59V617C150 609 154 606 146 481L132 275C124 170 117 125 112 93C105 50 84 39 27 34V0H267V34C200 39 176 48 173 93C170 130 171 172 176 256L190 540H192L414 8H438L682 554H685L689 152C689 45 686 41 604 33V0H871Z" id="g58-75"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="902" vert-adv-y="902" xpluscharwidth="38.48117294"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,38.426,3.818)"><path d="M700 305H441V272C542 264 548 258 548 186V107C548 64 536 53 516 42C493 30 462 24 429 24C237 24 151 189 151 333C151 517 264 626 415 626C507 626 585 594 609 470L642 476C634 544 629 601 626 636C594 644 513 665 429 665C234 665 46 554 46 321C46 123 194 -15 415 -15C495 -15 578 7 642 22C635 50 635 81 635 116V201C635 259 639 264 700 272V305Z" id="g58-69"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="725" vert-adv-y="725" xpluscharwidth="45.023478250000004"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,45.024,3.818)"><path d="M389 0V32C297 38 291 46 291 118V635C234 613 175 595 109 583V556L161 554C203 552 207 547 207 497V118C207 46 201 38 110 32V0H389Z" id="g50-50"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="487" vert-adv-y="487" xpluscharwidth="49.45568539"></glyph.data></g></svg> xylanase activity and stability. The xylanase activity at the starting time and without organic solvent added was taken as 100%. The data were presented as mean ± SD (<svg height="8.55521pt" id="M95" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="502" vert-adv-y="502" xpluscharwidth="6.526"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587" xpluscharwidth="17.789"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 635 136 610 108 579L65 516L85 496C110 533 150 575 205 575C258 575 300 543 300 481C300 407 232 369 141 339L147 310C163 315 188 321 211 321C268 321 338 284 338 192C338 94 288 40 217 40C160 40 119 68 93 91C85 98 77 97 69 91C60 84 47 71 46 58C44 46 48 35 62 22C75 10 116 -12 162 -12C234 -12 424 62 424 224C424 297 373 359 285 376V378Z" id="g113-52"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480" xpluscharwidth="27.661"></glyph.data></g></svg>) (for experimental details, please see the main text).</div>

International Journal of Microbiology

fig8

Figure 8

Figure 8: Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome 