Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome

Table 2

Effect of metal ions, reducing agents, and ionic and nonionic surfactants on xylanase activity.

Agent	2 mM	10 mM
None	100 ± 0.2	100 ± 0.2
Mg^2+	96.8 ± 0.9	92.4 ± 0.7
Ca^2+	91.4 ± 3.1	77.3 ± 2.9
Mn^2+	99.9 ± 2.5	97.3 ± 3.7
Co^2+	98.5 ± 2.9	94.2 ± 2.2
Cu^2+	86.3 ± 2.2	41.4 ± 1.9
Zn^2+	98.8 ± 0.9	95.5 ± 1.4
β-Mercaptoethanol	95.3 ± 1.0	93.6 ± 1.1
DTT	95.0 ± 1.6	94.7 ± 2.0
EDTA	91.6 ± 3.1	87.3 ± 3.1
SDS	22.1 ± 1.5	14.7 ± 1.3
Triton X-100	96.2 ± 2.2	103.9 ± 1.2
Tween- 80	97.9 ± 2.8	108.8 ± 2.6

The purified was assayed in the standard assay condition in the presence of 2 mM or 10 mM test agents. The xylanase activity measured in the absence of the test agent was set as 100%. All the values are means of three replications.