Research Article

Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies

Figure 4

Effect of (a) agitation and (b) inoculum size on D(−) lactic acid production and sucrose consumption by S. nakayamae. Culture conditions: GYP modified medium (120 g/L crystallized sugar, 35 g/L yeast extract, and 7.5 mL/L salt solution) with NaOH 10 N as pH neutralizing agent. (a) GYP modified medium at 35°C, pH 6.0, with 10% inoculum; (b) GYP modified medium at 35°C and pH 6.0 under 125 rpm.
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