Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine

Table 1

Summary of the target genes screened for using polymerase chain reaction (PCR).

Target gene	Primer name	Sequence (5′ to 3′)	Annealing temperature (°C)	Amplicon size; (bp)
Class D carbapenemases	OXA-23	F: GATCGGATTGGAGAACCAGA	58	501
		R: ATTTCTGACCGCATTTCCAT
	OXA-24	F: GGTTAGTTGGCCCCCTTAAA	58	246
		R: AGTTGAGCGAAAAGGGGATT
	OXA-58	F: AAGTATTGGGGCTTGTGCTG	58	599
		R: CCCCTCTGCGCTCTACATAC
	OXA-51	F: TAATGCTTTGATCGGCCTTG	58	353
		R: TGGATTGCACTTCATCTTGG
	OXA-143	F: TGGCACTTTCAGCAGTTCCT	58	180
		R: TAATCTTGAGGGGGCCAACC
	OXA-235	F: TTGTTGCCTTTACTTAGTTGC	58	700
		R: CAAAATTTTAAGACGGATCG
Class B carbapenemases	NDM	F: CCAATATTATGCACCCGGTCG	58	812
		R: ATGCGGGCCGTATGAGTGATTG
Class A carbapenemases	KPC	F: ATGTCACTGTATCGCCGTCT	58	538
		R: TTTTCAGAGCCTTACTGCCC
AME’s	aphA6	F: ATGGAATTGCCCAATATTATTC	55	797
		R: TCAATTCAATTCATCAAGTTTTA
	aphA1	F: CAACGGGAAACGTCTTGCTC	58	455
		R: ATTCGTGATTGCGCCTGAG
	aacA4	F: ATGACTGAGCATGACCTTGCG	65	518
		R: TTAGGCATCACTGCGTGTTCG
	aadB	F: ATGGACACAACGCAGGTCGC	65	524
		R: TTAGGCCGCATATCGCGACC
	aadA1	F: ATGAGGGAAGCGGTGATCG	65	254
		R: TTATTTGCCGACTACCTTGGTG
	aacC1	F: ATGGGCATCATTCGCACATGTAGG	65	456
		R: TTAGGTGGCGGTACTTGGGTC
Biofilm-producing virulence factors	ompA	F: CGCTTCTGCTGGTGCTGAAT	58	531
		R: CGTGCAGTAGCGTTAGGGTA
	epsA	F: AGCAAGTGGTTATCCAATCG	58	451
		R: ACCAGACTCACCCATTACAT

Annealing temperature (°C) used in this study.