Research Article
Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine
Table 1
Summary of the target genes screened for using polymerase chain reaction (PCR).
| Target gene | Primer name | Sequence (5′ to 3′) | Annealing temperature (°C) | Amplicon size (bp) |
| Class D carbapenemases | OXA-23 | F: GATCGGATTGGAGAACCAGA | 58 | 501 | R: ATTTCTGACCGCATTTCCAT | OXA-24 | F: GGTTAGTTGGCCCCCTTAAA | 58 | 246 | R: AGTTGAGCGAAAAGGGGATT | OXA-58 | F: AAGTATTGGGGCTTGTGCTG | 58 | 599 | R: CCCCTCTGCGCTCTACATAC | OXA-51 | F: TAATGCTTTGATCGGCCTTG | 58 | 353 | R: TGGATTGCACTTCATCTTGG | OXA-143 | F: TGGCACTTTCAGCAGTTCCT | 58 | 180 | R: TAATCTTGAGGGGGCCAACC | OXA-235 | F: TTGTTGCCTTTACTTAGTTGC | 58 | 700 | R: CAAAATTTTAAGACGGATCG |
| Class B carbapenemases | NDM | F: CCAATATTATGCACCCGGTCG | 58 | 812 | R: ATGCGGGCCGTATGAGTGATTG |
| Class A carbapenemases | KPC | F: ATGTCACTGTATCGCCGTCT | 58 | 538 | R: TTTTCAGAGCCTTACTGCCC |
| AME’s | aphA6 | F: ATGGAATTGCCCAATATTATTC | 55 | 797 | R: TCAATTCAATTCATCAAGTTTTA | aphA1 | F: CAACGGGAAACGTCTTGCTC | 58 | 455 | R: ATTCGTGATTGCGCCTGAG | aacA4 | F: ATGACTGAGCATGACCTTGCG | 65 | 518 | R: TTAGGCATCACTGCGTGTTCG | aadB | F: ATGGACACAACGCAGGTCGC | 65 | 524 | R: TTAGGCCGCATATCGCGACC | aadA1 | F: ATGAGGGAAGCGGTGATCG | 65 | 254 | R: TTATTTGCCGACTACCTTGGTG | aacC1 | F: ATGGGCATCATTCGCACATGTAGG | 65 | 456 | R: TTAGGTGGCGGTACTTGGGTC |
| Biofilm-producing virulence factors | ompA | F: CGCTTCTGCTGGTGCTGAAT | 58 | 531 | R: CGTGCAGTAGCGTTAGGGTA | epsA | F: AGCAAGTGGTTATCCAATCG | 58 | 451 | R: ACCAGACTCACCCATTACAT |
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Annealing temperature (°C) used in this study.
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